YY1 transcriptional initiator: Protein interactions and association with a DNA site containing unpaired strands

Anny Usheva, Thomas Shenk

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

The Ying-Yang 1 protein (YY1) DNA-binding site functions as an initiator element at which YY1, transcription factor IIb (TFIIB), and RNA polymerase II sponsor basal transcription from a supercoiled DNA template. We show that TFIIB hinds to YY1, stabilizing its interaction with DNA, and YY1 contacts the large subunit of polymerase II, directing it to the initiation site. YY1 directs initiation from linear DNA containing mismatched sequences within its binding site, leading us to infer that supercoiling facilitates the separation of DNA strands and to suggest that YY1 likely remains bound to the start site as DNA strands separate during initiation. These results provide a mechanistic basis for transcriptional initiation directed by YY1 in the absence of the TATA box-binding protein.

Original languageEnglish (US)
Pages (from-to)13571-13576
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number24
DOIs
StatePublished - Nov 26 1996

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • transcription factor IIB
  • transcription factor YY1
  • transcription initiation

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