X-gal staining of electroporated sea squirt (Ciona) embryos

Lionel Christiaen; Eileen Wagner; Weiyang Shi; Michael Levine

doi:10.1101/pdb.prot5346

X-gal staining of electroporated sea squirt (Ciona) embryos

Lionel Christiaen
, Eileen Wagner
, Weiyang Shi
, Michael Levine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.

Original language	English (US)
Journal	Cold Spring Harbor Protocols
Volume	4
Issue number	12
DOIs	https://doi.org/10.1101/pdb.prot5346
State	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot5346

Cite this

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title = "X-gal staining of electroporated sea squirt (Ciona) embryos",

abstract = "This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.",

author = "Lionel Christiaen and Eileen Wagner and Weiyang Shi and Michael Levine",

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AU - Christiaen, Lionel

AU - Wagner, Eileen

AU - Shi, Weiyang

AU - Levine, Michael

PY - 2009

Y1 - 2009

N2 - This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.

AB - This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.

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UR - https://www.scopus.com/pages/publications/72149107101#tab=citedBy

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DO - 10.1101/pdb.prot5346

M3 - Article

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JO - Cold Spring Harbor Protocols

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X-gal staining of electroporated sea squirt (Ciona) embryos

Abstract

All Science Journal Classification (ASJC) codes

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