Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences

Li Ye, William E. Allen, Kimberly R. Thompson, Qiyuan Tian, Brian Hsueh, Charu Ramakrishnan, Ai Chi Wang, Joshua H. Jennings, Avishek Adhikari, Casey H. Halpern, Ilana B. Witten, Alison L. Barth, Liqun Luo, Jennifer A. McNab, Karl Deisseroth

Research output: Contribution to journalArticlepeer-review

222 Scopus citations


A major challenge in understanding the cellular diversity of the brain has been linking activity during behavior with standard cellular typology. For example, it has not been possible to determine whether principal neurons in prefrontal cortex active during distinct experiences represent separable cell types, and it is not known whether these differentially active cells exert distinct causal influences on behavior. Here, we develop quantitative hydrogel-based technologies to connect activity in cells reporting on behavioral experience with measures for both brain-wide wiring and molecular phenotype. We find that positive and negative-valence experiences in prefrontal cortex are represented by cell populations that differ in their causal impact on behavior, long-range wiring, and gene expression profiles, with the major discriminant being expression of the adaptation-linked gene NPAS4. These findings illuminate cellular logic of prefrontal cortex information processing and natural adaptive behavior and may point the way to cell-type-specific understanding and treatment of disease-associated states.

Original languageEnglish (US)
Pages (from-to)1776-1788
Number of pages13
Issue number7
StatePublished - Jun 16 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences'. Together they form a unique fingerprint.

Cite this