@article{cc2c4370f1eb4a7889785b73ff769cc2,
title = "Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences",
abstract = "A major challenge in understanding the cellular diversity of the brain has been linking activity during behavior with standard cellular typology. For example, it has not been possible to determine whether principal neurons in prefrontal cortex active during distinct experiences represent separable cell types, and it is not known whether these differentially active cells exert distinct causal influences on behavior. Here, we develop quantitative hydrogel-based technologies to connect activity in cells reporting on behavioral experience with measures for both brain-wide wiring and molecular phenotype. We find that positive and negative-valence experiences in prefrontal cortex are represented by cell populations that differ in their causal impact on behavior, long-range wiring, and gene expression profiles, with the major discriminant being expression of the adaptation-linked gene NPAS4. These findings illuminate cellular logic of prefrontal cortex information processing and natural adaptive behavior and may point the way to cell-type-specific understanding and treatment of disease-associated states.",
author = "Li Ye and Allen, {William E.} and Thompson, {Kimberly R.} and Qiyuan Tian and Brian Hsueh and Charu Ramakrishnan and Wang, {Ai Chi} and Jennings, {Joshua H.} and Avishek Adhikari and Halpern, {Casey H.} and Witten, {Ilana B.} and Barth, {Alison L.} and Liqun Luo and McNab, {Jennifer A.} and Karl Deisseroth",
note = "Funding Information: We thank M. Greenberg (Harvard) for the NPAS4 antibody, E. Rosen (Harvard) for ribosome tag mice, N. Renier and M. Tessier-Lavigne (Rockefeller) for valuable discussions during lab visits, Christina Kim for discussions, and S. Pak, M. Lo, and C. Perry for technical assistance. W.E.A. is supported by a Fannie & John Hertz Foundation Fellowship. K.D. is supported by NIMH, NIDA, the Wiegers Family Fund, HHMI (HCIA support to L.L. and K.D.), and the U.S. Army Research Laboratory and Defense Advanced Research Projects Agency (Cooperative Agreement Number W911NF-14-2-0013); nothing in this material represents the official views or policies of these funders. Funding Information: We thank M. Greenberg (Harvard) for the NPAS4 antibody, E. Rosen (Harvard) for ribosome tag mice, N. Renier and M. Tessier-Lavigne (Rockefeller) for valuable discussions during lab visits, Christina Kim for discussions, and S. Pak, M. Lo, and C. Perry for technical assistance. W.E.A. is supported by a Fannie & John Hertz Foundation Fellowship. K.D. is supported by NIMH, NIDA, the Wiegers Family Fund, HHMI (HCIA support to L.L. and K.D.), and the U.S. Army Research Laboratory and Defense Advanced Research Projects Agency (Cooperative Agreement Number W911NF-14-2-0013); nothing in this material represents the official views or policies of these funders. The authors have disclosed these findings to the Stanford Office of Technology Licensing. K.D. is a founder and scientific advisor of ClearLight Diagnostics, a startup exploring improvement of cancer diagnostics using CLARITY-related methods; all software, clones, sequences, protocols, and computational resources are freely distributed and supported ( http://capture-clarity.org , http://clarityresourcecenter.org , and http://www.stanford.edu/group/dlab/optogenetics ). Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = jun,
day = "16",
doi = "10.1016/j.cell.2016.05.010",
language = "English (US)",
volume = "165",
pages = "1776--1788",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "7",
}