Wild-type p53 binds to the TATA-binding protein and represses transcription

Edward Seto, Anny Usheva, Gerard P. Zambetti, Jamil Momand, Nobuo Horikoshi, Roberto Weinmann, Arnold J. Levine, Thomas Shenk

Research output: Contribution to journalArticlepeer-review

535 Scopus citations


p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Original languageEnglish (US)
Pages (from-to)12028-12032
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number24
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • General


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