TY - JOUR
T1 - Widespread changes in the translation and adenylation of maternal messenger RNAs following fertilization of Spisula oocytes
AU - Rosenthal, Eric T.
AU - Ruderman, Joan V.
N1 - Funding Information:
We thank Despina Alexandraki, Terese Tansey, and Fred Wilt for their assistance and advice. This work was supported by National Science Foundation Grant PCM82-16917, a Research Career Development Award to J.V.R. (K04HD00349), and a National Institutes of Health predoctoral training grant to E.T.R.
PY - 1987/5
Y1 - 1987/5
N2 - We have reported previously that sequence-specific adenylations and deadenylations accompany changes in the translation of maternal mRNA following fertilization of Spisula oocytes (E. T. Rosenthal, T. R. Tansey, and J. V. Ruderman, 1983, J. Mol. Biol. 166, 309-327). The data presented here confirm and extend those observations. We have identified four classes of maternal mRNA with respect to translation: Class 1-not translated in oocytes and translated at very high efficiency immediately after fertilization, Class 2-not translated in oocytes and partially utilized for translation following fertilization, Class 3-translated in oocytes and not translated in embryos, and Class 4-not translated either before or after fertilization. There is an excellent, although not perfect, correlation between the translation of an mRNA and its polyadenylation status. The poly(A) tails of all the mRNAs which are translated in oocytes and untranslated in embryos are shortened at fertilization, and the poly(A) tails of those mRNAs which are untranslated in oocytes and translated in embryos are lengthened at fertilization. These adenylations and deadenylations occur simultaneously during the first 20 min following fertilization.
AB - We have reported previously that sequence-specific adenylations and deadenylations accompany changes in the translation of maternal mRNA following fertilization of Spisula oocytes (E. T. Rosenthal, T. R. Tansey, and J. V. Ruderman, 1983, J. Mol. Biol. 166, 309-327). The data presented here confirm and extend those observations. We have identified four classes of maternal mRNA with respect to translation: Class 1-not translated in oocytes and translated at very high efficiency immediately after fertilization, Class 2-not translated in oocytes and partially utilized for translation following fertilization, Class 3-translated in oocytes and not translated in embryos, and Class 4-not translated either before or after fertilization. There is an excellent, although not perfect, correlation between the translation of an mRNA and its polyadenylation status. The poly(A) tails of all the mRNAs which are translated in oocytes and untranslated in embryos are shortened at fertilization, and the poly(A) tails of those mRNAs which are untranslated in oocytes and translated in embryos are lengthened at fertilization. These adenylations and deadenylations occur simultaneously during the first 20 min following fertilization.
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U2 - 10.1016/0012-1606(87)90155-2
DO - 10.1016/0012-1606(87)90155-2
M3 - Article
C2 - 3569660
AN - SCOPUS:0023336496
SN - 0012-1606
VL - 121
SP - 237
EP - 246
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -