Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos

Lionel Christiaen; Eileen Wagner; Weiyang Shi; Michael Levine

doi:10.1101/pdb.prot5348

Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos

Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.

Original language	English (US)
Journal	Cold Spring Harbor Protocols
Volume	4
Issue number	12
DOIs	https://doi.org/10.1101/pdb.prot5348
State	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot5348

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abstract = "This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.",

author = "Lionel Christiaen and Eileen Wagner and Weiyang Shi and Michael Levine",

note = "Funding Information: This work was partially supported by the Ministry of Science and Technology of Taiwan under Project No. 103-2221-E-005 -010 -MY3.",

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AU - Christiaen, Lionel

AU - Wagner, Eileen

AU - Shi, Weiyang

AU - Levine, Michael

N1 - Funding Information: This work was partially supported by the Ministry of Science and Technology of Taiwan under Project No. 103-2221-E-005 -010 -MY3.

PY - 2009

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N2 - This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.

AB - This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.

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Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos

Abstract

All Science Journal Classification (ASJC) codes

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