Whole-genome ChIP-chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo

Julia Zeitlinger, Robert P. Zinzen, Alexander Stark, Manolis Kellis, Hailan Zhang, Richard A. Young, Michael Levine

Research output: Contribution to journalArticlepeer-review

221 Scopus citations

Abstract

Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of the dorsoventral (DV) determinants Dorsal, Twist, and Snail in the Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP-chip). The in vivo binding of these proteins correlate tightly with the limits of known enhancers. Our analysis predicts substantially more target genes than previous estimates, and includes Dpp signaling components and anteroposterior (AP) segmentation determinants. Thus, the ChIP-chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development.

Original languageEnglish (US)
Pages (from-to)385-390
Number of pages6
JournalGenes and Development
Volume21
Issue number4
DOIs
StatePublished - Feb 15 2007

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Chip-chip
  • Dorsoventral patterning
  • Gene regulatory networks
  • Segmentation

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