TY - JOUR
T1 - Whole-genome analysis of Dorsal-ventral patterning in the Drosophila embryo
AU - Stathopoulos, Angelike
AU - Van Drenth, Madeleine
AU - Erives, Albert
AU - Markstein, Michele
AU - Levine, Michael
N1 - Funding Information:
We thank M. Blanchette, A. Hochheimer, E. Laborier, D. Rio, and R. Tjian for advice and technical assistance with the microarray experiments, and L. Mirels and R. Zinzen for critically reading the manuscript. We also thank R. Zinzen for help with Figure 5M and K. Senger for sharing SELEX data. P. Markstein independently identified the CACATGT motif using a software package, Mermaid, that is under development. We are grateful to H. Ashe for the Kr-FSF expression vector and probes and Y. Nibu for providing ESTs. This work was funded by a grant from the NIH (GM46638) to M.L. A.S. is supported by a postdoctoral fellowship from the NIH (GM20352).
PY - 2002/11/27
Y1 - 2002/11/27
N2 - The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tissue-specific form of the NF-Y transcription complex is essential for the activation of gene expression in the mesoderm. Tissue-specific enhancers were identified for new Dorsal target genes, and bioinformatics methods identified conserved cis-regulatory elements for coordinately regulated genes that respond to similar thresholds of the Dorsal gradient. The new Dorsal target genes and enhancers represent one of the most extensive gene networks known for any developmental process.
AB - The maternal Dorsal regulatory gradient initiates the differentiation of several tissues in the early Drosophila embryo. Whole-genome microarray assays identified as many as 40 new Dorsal target genes, which encode a broad spectrum of cell signaling proteins and transcription factors. Evidence is presented that a tissue-specific form of the NF-Y transcription complex is essential for the activation of gene expression in the mesoderm. Tissue-specific enhancers were identified for new Dorsal target genes, and bioinformatics methods identified conserved cis-regulatory elements for coordinately regulated genes that respond to similar thresholds of the Dorsal gradient. The new Dorsal target genes and enhancers represent one of the most extensive gene networks known for any developmental process.
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U2 - 10.1016/S0092-8674(02)01087-5
DO - 10.1016/S0092-8674(02)01087-5
M3 - Article
C2 - 12464180
AN - SCOPUS:0037184519
SN - 0092-8674
VL - 111
SP - 687
EP - 701
JO - Cell
JF - Cell
IS - 5
ER -