TY - JOUR
T1 - Visualizing hepatitis C virus infection in humanized mice
AU - von Schaewen, Markus
AU - Ding, Qiang
AU - Ploss, Alexander
N1 - Funding Information:
The authors thank Brigitte Heller and Benjamin Winer for edits and critical discussion of the manuscript. Work in the laboratory is supported in part by grants from the National Institutes of Health ( 2 R01 AI079031-05A1 , 1 R01 AI107301-01 , 1 R56 AI106005-01 , 1R21 AI 106000–01 ), the Walter Reed Army Institute of Research , the Bill and Melinda Gates Foundation and the Grand Challenges Program at Princeton University . M.v.S. is a recipient of a fellowship from the German Research Foundation (Deutsche Forschungsgemeinschaft) .
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Hepatitis C virus (HCV) establishes frequently persistent infections. Chronic carriers can develop severe liver disease. HCV has been intensely studied in a variety of cell culture systems. However, commonly used cell lines and primary hepatocyte cultures do not or only in part recapitulate the intricate host environment HCV faces in the liver. HCV infects readily only humans and chimpanzees, which poses challenges in studying HCV infection in vivo. Consequently, tractable small animal models are needed that are not only suitable for analyzing HCV infection but also for testing novel therapeutics. Here, we will focus our discussion on humanized mice, i.e. mice engrafted with human tissues or expressing human genes, which support HCV infection. We will further highlight novel methods that can be used to unambiguously detect HCV infected cells in situ, thereby facilitating a spatio-temporal dissection of HCV infection in the three dimensional context of the liver.
AB - Hepatitis C virus (HCV) establishes frequently persistent infections. Chronic carriers can develop severe liver disease. HCV has been intensely studied in a variety of cell culture systems. However, commonly used cell lines and primary hepatocyte cultures do not or only in part recapitulate the intricate host environment HCV faces in the liver. HCV infects readily only humans and chimpanzees, which poses challenges in studying HCV infection in vivo. Consequently, tractable small animal models are needed that are not only suitable for analyzing HCV infection but also for testing novel therapeutics. Here, we will focus our discussion on humanized mice, i.e. mice engrafted with human tissues or expressing human genes, which support HCV infection. We will further highlight novel methods that can be used to unambiguously detect HCV infected cells in situ, thereby facilitating a spatio-temporal dissection of HCV infection in the three dimensional context of the liver.
KW - Animal model
KW - Hepatitis C virus
KW - Humanized mice
KW - Imaging
KW - Viral hepatitis
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U2 - 10.1016/j.jim.2014.03.006
DO - 10.1016/j.jim.2014.03.006
M3 - Article
C2 - 24642425
AN - SCOPUS:84908509736
SN - 0022-1759
VL - 410
SP - 50
EP - 59
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
ER -