Visualizing hepatitis C virus infection in humanized mice

Markus von Schaewen, Qiang Ding, Alexander Ploss

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Hepatitis C virus (HCV) establishes frequently persistent infections. Chronic carriers can develop severe liver disease. HCV has been intensely studied in a variety of cell culture systems. However, commonly used cell lines and primary hepatocyte cultures do not or only in part recapitulate the intricate host environment HCV faces in the liver. HCV infects readily only humans and chimpanzees, which poses challenges in studying HCV infection in vivo. Consequently, tractable small animal models are needed that are not only suitable for analyzing HCV infection but also for testing novel therapeutics. Here, we will focus our discussion on humanized mice, i.e. mice engrafted with human tissues or expressing human genes, which support HCV infection. We will further highlight novel methods that can be used to unambiguously detect HCV infected cells in situ, thereby facilitating a spatio-temporal dissection of HCV infection in the three dimensional context of the liver.

Original languageEnglish (US)
Pages (from-to)50-59
Number of pages10
JournalJournal of Immunological Methods
Volume410
DOIs
StatePublished - Aug 1 2014

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Keywords

  • Animal model
  • Hepatitis C virus
  • Humanized mice
  • Imaging
  • Viral hepatitis

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