Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

Lindsey E. McQuade, Jie Ma, Graeme Lowe, Ambarish Ghatpande, Alan Gelperin, Stephen J. Lippard

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.

Original languageEnglish (US)
Pages (from-to)8525-8530
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number19
DOIs
StatePublished - May 11 2010

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Fluorescence microscopy
  • Fluorescent sensing
  • NO
  • Olfaction
  • Trappable probe

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