Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

Lindsey E. McQuade; Jie Ma; Graeme Lowe; Ambarish Ghatpande; Alan Gelperin; Stephen J. Lippard

doi:10.1073/pnas.0914794107

Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

Lindsey E. McQuade, Jie Ma, Graeme Lowe, Ambarish Ghatpande, Alan Gelperin, Stephen J. Lippard

Princeton Neuroscience Institute

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu₂(FL2E) and the membrane-impermeable acid derivative Cu₂(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu₂(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.

Original language	English (US)
Pages (from-to)	8525-8530
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	107
Issue number	19
DOIs	https://doi.org/10.1073/pnas.0914794107
State	Published - May 11 2010

All Science Journal Classification (ASJC) codes

General

Keywords

Fluorescence microscopy
Fluorescent sensing
NO
Olfaction
Trappable probe

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10.1073/pnas.0914794107

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title = "Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe",

abstract = "We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.",

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doi = "10.1073/pnas.0914794107",

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Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe. / McQuade, Lindsey E.; Ma, Jie; Lowe, Graeme et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 19, 11.05.2010, p. 8525-8530.

Research output: Contribution to journal › Article › peer-review

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T1 - Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

AU - McQuade, Lindsey E.

AU - Ma, Jie

AU - Lowe, Graeme

AU - Ghatpande, Ambarish

AU - Gelperin, Alan

AU - Lippard, Stephen J.

PY - 2010/5/11

Y1 - 2010/5/11

N2 - We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.

AB - We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.

KW - Fluorescence microscopy

KW - Fluorescent sensing

KW - NO

KW - Olfaction

KW - Trappable probe

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Visualization of nitric oxide production in the mouse main olfactory bulb by a cell-trappable copper(II) fluorescent probe

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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