Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection

Thomas E. Shenk; Victor Stollar

doi:10.1016/0006-291X(72)90009-5

Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection

Thomas E. Shenk
, Victor Stollar

Molecular Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Only one species of double-stranded RNA (20S) is found in BHK-21 cells after infection with plaque-purified Sindbis virus. A smaller species of double-stranded RNA (12S), which is shown to contain viral sequences by hydridization experiments, is present when BHK-21 cells are infected at high multiplicities with a Sindbis virus stock derived by repeated passage at high multiplicity of infection. Several small single-stranded RNA species (0.9 to 2.2 × 10⁵ MW) not found in cells infected with plaque-purified virus are present in infected cells which contain 12S DS RNA.

Original language	English (US)
Pages (from-to)	60-67
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(72)90009-5
State	Published - Oct 6 1972

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(72)90009-5

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@article{532a7c1e454242d7adcc65beba1b17a7,

title = "Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection",

abstract = "Only one species of double-stranded RNA (20S) is found in BHK-21 cells after infection with plaque-purified Sindbis virus. A smaller species of double-stranded RNA (12S), which is shown to contain viral sequences by hydridization experiments, is present when BHK-21 cells are infected at high multiplicities with a Sindbis virus stock derived by repeated passage at high multiplicity of infection. Several small single-stranded RNA species (0.9 to 2.2 × 105 MW) not found in cells infected with plaque-purified virus are present in infected cells which contain 12S DS RNA.",

author = "Shenk, \{Thomas E.\} and Victor Stollar",

note = "Funding Information: ACKNOWLEDGEMENTWSe. would like to thank Dr. R. Walter Schlesinger for his commentsa nd suggestions, Dr. Victor Raso for performing the complement fixation assays, and Mrs. Aleksandra Semionowf or her technical assistance. This investigation was supported by the US-Japan Cooperative Medical Science Program administered by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (Grant AI-05920). T.E.S. was supported by a National Institutes of Health Predoctoral Fellowship (GM-48773). The work reported in this paper will be included in the dissertation to be submitted by T.E.S. in partial fulfillment of the requirements for the Ph.D. degree in Microbiology awarded by the Graduate School of Rutgers University.",

year = "1972",

month = oct,

day = "6",

doi = "10.1016/0006-291X(72)90009-5",

language = "English (US)",

volume = "49",

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journal = "Biochemical and Biophysical Research Communications",

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T1 - Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection

AU - Shenk, Thomas E.

AU - Stollar, Victor

N1 - Funding Information: ACKNOWLEDGEMENTWSe. would like to thank Dr. R. Walter Schlesinger for his commentsa nd suggestions, Dr. Victor Raso for performing the complement fixation assays, and Mrs. Aleksandra Semionowf or her technical assistance. This investigation was supported by the US-Japan Cooperative Medical Science Program administered by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (Grant AI-05920). T.E.S. was supported by a National Institutes of Health Predoctoral Fellowship (GM-48773). The work reported in this paper will be included in the dissertation to be submitted by T.E.S. in partial fulfillment of the requirements for the Ph.D. degree in Microbiology awarded by the Graduate School of Rutgers University.

PY - 1972/10/6

Y1 - 1972/10/6

N2 - Only one species of double-stranded RNA (20S) is found in BHK-21 cells after infection with plaque-purified Sindbis virus. A smaller species of double-stranded RNA (12S), which is shown to contain viral sequences by hydridization experiments, is present when BHK-21 cells are infected at high multiplicities with a Sindbis virus stock derived by repeated passage at high multiplicity of infection. Several small single-stranded RNA species (0.9 to 2.2 × 105 MW) not found in cells infected with plaque-purified virus are present in infected cells which contain 12S DS RNA.

AB - Only one species of double-stranded RNA (20S) is found in BHK-21 cells after infection with plaque-purified Sindbis virus. A smaller species of double-stranded RNA (12S), which is shown to contain viral sequences by hydridization experiments, is present when BHK-21 cells are infected at high multiplicities with a Sindbis virus stock derived by repeated passage at high multiplicity of infection. Several small single-stranded RNA species (0.9 to 2.2 × 105 MW) not found in cells infected with plaque-purified virus are present in infected cells which contain 12S DS RNA.

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DO - 10.1016/0006-291X(72)90009-5

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AN - SCOPUS:0015496149

SN - 0006-291X

VL - 49

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection

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