Viral RNA species in BHK-21 cells infected with sindbis virus serially passaged at high multiplicity of infection

Thomas E. Shenk, Victor Stollar

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Only one species of double-stranded RNA (20S) is found in BHK-21 cells after infection with plaque-purified Sindbis virus. A smaller species of double-stranded RNA (12S), which is shown to contain viral sequences by hydridization experiments, is present when BHK-21 cells are infected at high multiplicities with a Sindbis virus stock derived by repeated passage at high multiplicity of infection. Several small single-stranded RNA species (0.9 to 2.2 × 105 MW) not found in cells infected with plaque-purified virus are present in infected cells which contain 12S DS RNA.

Original languageEnglish (US)
Pages (from-to)60-67
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume49
Issue number1
DOIs
StatePublished - Oct 6 1972

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biophysics
  • Biochemistry
  • Cell Biology

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