Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-13C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface

Cory A. Lytle; Mark E. Fuller; Ying dong M. Gan; Aaron Peacock; Mary F. DeFlaun; Tullis C. Onstott; David C. White

doi:10.1016/S0167-7012(00)00245-1

Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-¹³C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface

Cory A. Lytle, Mark E. Fuller, Ying dong M. Gan, Aaron Peacock, Mary F. DeFlaun, Tullis C. Onstott, David C. White

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per ¹³C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (281+18) as negative ions in PG and PE separated by high performance liquid chromatography (HPLC) and detected after up-front collisionally induced dissociation (CID) utilizing electrospray (ES) mass spectrometry (MS) provided sufficient sensitivity and specificity for detection in the presence of the indigenous microbiota. Application of tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) was use to monitor selected transitions. MRM can increase the sensitivity so that polar lipids recovered from cell densities currently at about 10⁴ cells/sample can be detected. This technology provides a non-intrusive mechanism for monitoring the distribution of bacteria added to accelerate in situ bioremediation of subsurface sediments.

Original language	English (US)
Pages (from-to)	271-281
Number of pages	11
Journal	Journal of Microbiological Methods
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/S0167-7012(00)00245-1
State	Published - Apr 2 2001

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

Keywords

Electrospray ionization
Environmental detection of bacteria
High performance liquid chromatography/electrospray ionization/mass spectrometry
Per C-labeled fatty acids
Phospatidylethanolamine
Phosphatidylglycerol
Phospholipids

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10.1016/S0167-7012(00)00245-1

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Lytle, C. A., Fuller, M. E., Gan, Y. D. M., Peacock, A., DeFlaun, M. F., Onstott, T. C., & White, D. C. (2001). Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-¹³C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface. Journal of Microbiological Methods, 44(3), 271-281. https://doi.org/10.1016/S0167-7012(00)00245-1

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title = "Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-13C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface",

abstract = "Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (281+18) as negative ions in PG and PE separated by high performance liquid chromatography (HPLC) and detected after up-front collisionally induced dissociation (CID) utilizing electrospray (ES) mass spectrometry (MS) provided sufficient sensitivity and specificity for detection in the presence of the indigenous microbiota. Application of tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) was use to monitor selected transitions. MRM can increase the sensitivity so that polar lipids recovered from cell densities currently at about 104 cells/sample can be detected. This technology provides a non-intrusive mechanism for monitoring the distribution of bacteria added to accelerate in situ bioremediation of subsurface sediments.",

keywords = "Electrospray ionization, Environmental detection of bacteria, High performance liquid chromatography/electrospray ionization/mass spectrometry, Per C-labeled fatty acids, Phospatidylethanolamine, Phosphatidylglycerol, Phospholipids",

author = "Lytle, {Cory A.} and Fuller, {Mark E.} and Gan, {Ying dong M.} and Aaron Peacock and DeFlaun, {Mary F.} and Onstott, {Tullis C.} and White, {David C.}",

note = "Funding Information: This work was supported by National Science Foundation grant DEB 9814813 and the Department of Energy, Office of Energy Research, grant number DE-FC02-96ER62278White (part of the Assessment Component of the Natural and Accelerated Bioremediation Research Program, NABIR).",

year = "2001",

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doi = "10.1016/S0167-7012(00)00245-1",

language = "English (US)",

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pages = "271--281",

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Lytle, CA, Fuller, ME, Gan, YDM, Peacock, A, DeFlaun, MF, Onstott, TC & White, DC 2001, 'Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-¹³C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface', Journal of Microbiological Methods, vol. 44, no. 3, pp. 271-281. https://doi.org/10.1016/S0167-7012(00)00245-1

Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-¹³C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface. / Lytle, Cory A.; Fuller, Mark E.; Gan, Ying dong M. et al.
In: Journal of Microbiological Methods, Vol. 44, No. 3, 02.04.2001, p. 271-281.

Research output: Contribution to journal › Article › peer-review

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T1 - Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-13C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface

AU - Lytle, Cory A.

AU - Fuller, Mark E.

AU - Gan, Ying dong M.

AU - Peacock, Aaron

AU - DeFlaun, Mary F.

AU - Onstott, Tullis C.

AU - White, David C.

N1 - Funding Information: This work was supported by National Science Foundation grant DEB 9814813 and the Department of Energy, Office of Energy Research, grant number DE-FC02-96ER62278White (part of the Assessment Component of the Natural and Accelerated Bioremediation Research Program, NABIR).

PY - 2001/4/2

Y1 - 2001/4/2

N2 - Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (281+18) as negative ions in PG and PE separated by high performance liquid chromatography (HPLC) and detected after up-front collisionally induced dissociation (CID) utilizing electrospray (ES) mass spectrometry (MS) provided sufficient sensitivity and specificity for detection in the presence of the indigenous microbiota. Application of tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) was use to monitor selected transitions. MRM can increase the sensitivity so that polar lipids recovered from cell densities currently at about 104 cells/sample can be detected. This technology provides a non-intrusive mechanism for monitoring the distribution of bacteria added to accelerate in situ bioremediation of subsurface sediments.

AB - Specific fatty acids from phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) recovered from a per 13C-labeled bacteria can be detected in environmental samples and used as measures of bacterial transport in the subsurface. Detection of palmitic acid (16:0) and oleic acid (18:1) at m/z 271 (255+16) and 299 (281+18) as negative ions in PG and PE separated by high performance liquid chromatography (HPLC) and detected after up-front collisionally induced dissociation (CID) utilizing electrospray (ES) mass spectrometry (MS) provided sufficient sensitivity and specificity for detection in the presence of the indigenous microbiota. Application of tandem mass spectrometry (MS/MS) in the multiple reaction monitoring (MRM) was use to monitor selected transitions. MRM can increase the sensitivity so that polar lipids recovered from cell densities currently at about 104 cells/sample can be detected. This technology provides a non-intrusive mechanism for monitoring the distribution of bacteria added to accelerate in situ bioremediation of subsurface sediments.

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KW - Environmental detection of bacteria

KW - High performance liquid chromatography/electrospray ionization/mass spectrometry

KW - Per C-labeled fatty acids

KW - Phospatidylethanolamine

KW - Phosphatidylglycerol

KW - Phospholipids

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Lytle CA, Fuller ME, Gan YDM, Peacock A, DeFlaun MF, Onstott TC et al. Utility of high performance liquid chromatography/electrospray/mass spectrometry of polar lipids in specifically Per-¹³C labeled Gram-negative bacteria DA001 as a tracer for acceleration of bioremediation in the subsurface. Journal of Microbiological Methods. 2001 Apr 2;44(3):271-281. doi: 10.1016/S0167-7012(00)00245-1