Using RNA tags for multicolor live imaging of chromatin loci and transcription in drosophila embryos

Hongtao Chen, Thomas Gregor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Elucidating the biological implications of higher order chromatin architectures in animal development requires simultaneous, quantitative measurements of chromatin dynamics and transcriptional activity in living specimen. Here we describe a multicolor labeling and live imaging approach in embryos of the fruit fly Drosophila melanogaster. The method allows simultaneous measurement of movements of specific loci and their transcriptional activity for developmental genes, enabling new approaches to probe the interaction between 3D chromatin architecture and regulation of gene expression in development.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages373-384
Number of pages12
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2166
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • 3D genome architecture
  • Drosophila embryos
  • Quantitative live imaging
  • Transcription

Fingerprint Dive into the research topics of 'Using RNA tags for multicolor live imaging of chromatin loci and transcription in drosophila embryos'. Together they form a unique fingerprint.

  • Cite this

    Chen, H., & Gregor, T. (2020). Using RNA tags for multicolor live imaging of chromatin loci and transcription in drosophila embryos. In Methods in Molecular Biology (pp. 373-384). (Methods in Molecular Biology; Vol. 2166). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0712-1_22