Use of differential display and dna array technology to assess the effect of human cytomegalovirus infection on signal transduction pathways

Hua Zhu, Jiang Ping Cong, Gargi Mamtora, Thomas Gingeras, Thomas Shenk

Research output: Contribution to journalArticlepeer-review

Abstract

We employed differential display and DNA array analyses 10 identify cellular mRNAs that accumulate to enhanced or reduced levels in human cytornegalovirus-infected as compared to mock-infected cells. R\A> were com pared by differential display at 8 h after infection of primary human fibrohlasts. Partial cDNA clones were isolated corresponding to about '20 differentially e.x pressed cellular m R N As. Many of the cellular mRNAs thai accumulate to elevated levels are induced by interferon-alpha and beta. Neutralizing anlibod ies that block virus infection also block the induction. These RNAs accumulate after infection with virus that has been inactivated by treatment with UV light, indicating that the inducer is present in virions. We conclude t liât human cy tomegalovirus induces interferon-responsive mRNAs. and we are exploring the pathway that underlies the induction. More recently, we utilized DNA array technology to ascertain the effect of IICMV infection on the steady state level of approximately 6600 human mRNAs. At -10 minutes after infection, the level of about 25 cellular mRNAs is elevated or decreased, and the number of mRNAs whose level has changed increases to 00 and 360 at rt ami 2\ hours after infection, respectively. As expected, it is evident that the Interferon response pathway is activated, and we are analyzing additional classes of <ellular gene whose expression is modulated hy i he virus.

Original languageEnglish (US)
Pages (from-to)A1307
JournalFASEB Journal
Volume12
Issue number8
StatePublished - Dec 1 1998

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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