TY - JOUR
T1 - Unwinding the functions of the Pif1 family helicases
AU - Bochman, Matthew L.
AU - Sabouri, Nasim
AU - Zakian, Virginia A.
N1 - Funding Information:
We thank Beiyu Liu, Jiangyang Wang, and Paul Englund for sharing unpublished data and helpful discussions on the trypanosome helicases. NS was supported by the Wenner-Gren Foundation and MB by the institution training grant NIH 2 T32 CA00952. Research on Pif1 family helicases in the Zakian lab is supported by NIH RO1 GM026938.
PY - 2010/3/2
Y1 - 2010/3/2
N2 - Helicases are ubiquitous enzymes found in all organisms that are necessary for all (or virtually all) aspects of nucleic acid metabolism. The Pif1 helicase family is a group of 5′ → 3′ directed, ATP-dependent, super family IB helicases found in nearly all eukaryotes. Here, we review the discovery, evolution, and what is currently known about these enzymes in Saccharomyces cerevisiae (ScPif1 and ScRrm3), Schizosaccharomyces pombe (SpPfh1), Trypanosoma brucei (TbPIF1, 2, 5, and 8), mice (mPif1), and humans (hPif1). Pif1 helicases variously affect telomeric, ribosomal, and mitochondrial DNA replication, as well as Okazaki fragment maturation, and in at least some cases affect these processes by using their helicase activity to disrupt stable nucleoprotein complexes. While the functions of these enzymes vary within and between organisms, it is evident that Pif1 family helicases are crucial for both nuclear and mitochondrial genome maintenance.
AB - Helicases are ubiquitous enzymes found in all organisms that are necessary for all (or virtually all) aspects of nucleic acid metabolism. The Pif1 helicase family is a group of 5′ → 3′ directed, ATP-dependent, super family IB helicases found in nearly all eukaryotes. Here, we review the discovery, evolution, and what is currently known about these enzymes in Saccharomyces cerevisiae (ScPif1 and ScRrm3), Schizosaccharomyces pombe (SpPfh1), Trypanosoma brucei (TbPIF1, 2, 5, and 8), mice (mPif1), and humans (hPif1). Pif1 helicases variously affect telomeric, ribosomal, and mitochondrial DNA replication, as well as Okazaki fragment maturation, and in at least some cases affect these processes by using their helicase activity to disrupt stable nucleoprotein complexes. While the functions of these enzymes vary within and between organisms, it is evident that Pif1 family helicases are crucial for both nuclear and mitochondrial genome maintenance.
KW - DNA replication
KW - Helicase
KW - Pfh1p
KW - Pif1
KW - Rrm3
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U2 - 10.1016/j.dnarep.2010.01.008
DO - 10.1016/j.dnarep.2010.01.008
M3 - Review article
C2 - 20097624
AN - SCOPUS:76749129611
SN - 1568-7864
VL - 9
SP - 237
EP - 249
JO - DNA Repair
JF - DNA Repair
IS - 3
ER -