@article{c61749fca8ab4be09ca4d6220180d6c0,
title = "Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility",
abstract = "The predatory deltaproteobacterium Myxococcus xanthus uses a helically-trafficked motor at bacterial focaladhesion (bFA) sites to power gliding motility. Using total internal reflection fluorescence and force microscopies, we identify the von Willebrand A domain-containing outer-membrane (OM) lipoprotein CglB as an essential substratum-coupling adhesin of the gliding transducer (Glt) machinery at bFAs. Biochemical and genetic analyses reveal that CglB localizes to the cell surface independently of the Glt apparatus; once there, it is recruited by the OM module of the gliding machinery, a heteroligomeric complex containing the integral OM β barrels GltA, GltB, and GltH, as well as the OM protein GltC and OM lipoprotein GltK. This Glt OM platform mediates the cell-surface accessibility and retention of CglB by the Glt apparatus. Together, these data suggest that the gliding complex promotes regulated surface exposure of CglB at bFAs, thus explaining the manner by which contractile forces exerted by inner-membrane motors are transduced across the cell envelope to the substratum.",
author = "Islam, {Salim T.} and Jolivet, {Nicolas Y.} and Cl{\'e}mence Cuzin and Belgrave, {Akeisha M.} and Laetitia My and Betty Fleuchot and Faure, {Laura M.} and Utkarsha Mahanta and Kezzo, {Ahmad A.} and Fares Sa{\"i}di and Gaurav Sharma and Fiche, {Jean Bernard} and Bratton, {Benjamin P.} and Julien Herrou and Marcelo Nollmann and Shaevitz, {Joshua W.} and Eric Durand and T{\^a}m Mignot",
note = "Funding Information: This work was funded by the Natural Sciences and Engineering Research Council of Canada, discovery grant RGPIN-2016-06637 (to S.T.I., N.Y.J., and F.S.); the Banting Research Foundation, Discovery Award 2018-1400 (to S.T.I.); the Canadian Institutes of Health Research fellowship (to S.T.I.); the Aix-Marseille University AMIDEX Excellence Program (to S.T.I.); PROTEO, The Quebec Network for Research on Protein Function, Engineering, and Applications studentship (to N.Y.J. and F.S.); the European Research Council, advanced grant JAWS (to T.M.); the Bettencourt-Schueller Foundation, Coup d'{\'e}lan pour la recherche fran{\c c}aise 2011 (to T.M.); the National Science Foundation, grant no. CAREER PHY-0844466 (to J.W.S.); the Fondation ARC studentship (to L.M.F.); the Glenn Centers for Aging Research award (to B.P.B.); and the NIH award P50 GM071508 (to B.P.B.). Funding Information: Acknowledgments:W ewouldliketothankJ.SelkrigandN.Nickersonforvaluablesuggestions and troubleshooting regarding protease accessibility; A. Konovalova for constructive input on surfacelipoproteins;R.Fieldhouseforinsightfuldiscussionsonproteinmodelingand evolutionarycouplings;S.GauthierforcloningpSWU30-pCglBWT;L.S{\o}gaard-Andersenforthe giftofα-GltK,α-GltB,α-GltA,andα-GltCpolyclonalantibodies;A.RousselandR.Vincentelli (CNRS–Aix-MarseilleUniversity,Architectureetfunctiondesmacromoleculesbiologiques)for CglBproteinusedtogeneratepAb;R.Lebrun,P .Mansuelle,andR.PuppooftheMediterranean Institute of Microbiology{\textquoteright}s Proteomics Platform for insightful discussions and sample processingformassspectrometry;andE.MaurielloforinvaluableassistancewithE.coli immunofluorescencelabelingexperiments.Funding:ThisworkwasfundedbytheNatural SciencesandEngineeringResearchCouncilofCanada,discoverygrantRGPIN-2016-06637(to S.T .I., N.Y .J., andF .S.); theBantingResearchFoundation,Discov ery A ward2018-1400(toS.T .I.); theCanadianInstitutesofHealthResearchfellowship(toS.T .I.); theAix-MarseilleUniversity AMIDEXExcellenceProgram(toS.T .I.); PRO TEO, TheQuebecNetworkforResearchonProtein Function,Engineering,andApplicationsstudentship(toN.Y .J. andF .S.); theEuropeanResearch Council, advanced grant JA WS (to T .M.); the Bettencourt-Schueller Foundation, Coup d{\textquoteright}{\'e}lan pour la recherche fran{\c c}aise 2011 (to T .M.); the National Science Foundation, grant no. CAREER PHY-0844466(toJ.W .S.); theFondationARCstudentship(toL.M.F .); theGlennCentersforAging Researchaward(toB.P .B.); andtheNIHawardP50GM071508(toB.P .B.). Authorcontributions: Conceptualization:S.T .I., N.Y .J., C.C.,E.D.,andT .M. Methodology:S.T .I., N.Y .J., C.C.,A.M.B.,L.M., J.H.,E.D.,andT .M. Investigation:S.T .I., N.Y .J., C.C.,E.D.,A.M.B.,L.M.,U.M.,B.F ., L.M.F ., G.S.,A.A.K., F .S., J.-B.F ., B.P .B., andJ.H.Visualization:S.T .I., N.Y .J., G.S.,J.H.,andT .M. Supervision:S.T .I., T .M., J.W .S., M.N., E.D., and G.S. Writing—original draft: S.T .I., N.Y .J., and T .M. Writing—reviewand editing:S.T .I., T .M., N.Y .J., J.W .S., G.S.,andM.N.Competinginterests:Theauthorsdeclarethat theyhav enocompetinginterests.Dataandmaterialsavailability:Alldataneededto evaluatetheconclusionsinthepaperarepresentinthepaperand/ortheSupplementary Materials. Publisher Copyright: {\textcopyright} 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science.",
year = "2023",
month = feb,
doi = "10.1126/sciadv.abq0619",
language = "English (US)",
volume = "9",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "8",
}