Uncoupling of influenza a virus transcription and replication through mutation of the unpaired adenosine in the viral RNA promoter

Aartjan J.W. te Velthuis, Lauren Turrell, Frank T. Vreede, Ervin Fodor

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Transcription and replication of the influenza A virus RNA genome are mediated by the viral RNA polymerase from a promoter consisting of the partially base-paired 3= and 5= termini of viral genome segments. Here we show that transcription and replication can be uncoupled by mutation of an unpaired adenosine in the 5' strand of the promoter. This residue is important for transcription but not replication by being essential for the cap-binding activity of the RNA polymerase.

Original languageEnglish (US)
Pages (from-to)10381-10384
Number of pages4
JournalJournal of virology
Volume87
Issue number18
DOIs
StatePublished - 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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