Uncoupling neurogenic gene networks in the drosophila embryo

William A. Rogers, Yogesh Goyal, Kei Yamaya, Stanislav Y. Shvartsman, Michael S. Levine

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind).EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand,which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.

Original languageEnglish (US)
Pages (from-to)634-638
Number of pages5
JournalGenes and Development
Issue number7
StatePublished - Apr 1 2017

All Science Journal Classification (ASJC) codes

  • General Medicine


  • Central nervous system
  • Drosophila embryo
  • EGFR regulatory networks
  • Intermediate neuroblasts defective
  • Rhomboid


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