Ultrafast protein splicing is common among cyanobacterial split inteins: Implications for protein engineering

Neel H. Shah, Geoffrey P. Dann, Miquel Vila-Perelló, Zhihua Liu, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

We describe the first systematic study of a family of inteins, the split DnaE inteins from cyanobacteria. By measuring in vivo splicing efficiencies and in vitro kinetics, we demonstrate that several inteins can catalyze protein trans-splicing in tens of seconds rather than hours, as is commonly observed for this autoprocessing protein family. Furthermore, we show that when artificially fused, these inteins can be used for rapid generation of protein α-thioesters for expressed protein ligation. This comprehensive survey of split inteins provides indispensable information for the development and improvement of intein-based tools for chemical biology.

Original languageEnglish (US)
Pages (from-to)11338-11341
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number28
DOIs
StatePublished - Jul 18 2012

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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