Abstract
Specifying the location of cells with respect to each other is important for cell-based biosensors, tissue engineering, and fundamental questions in cell biology. Cell-substrate interactions can be spatially controlled using a variety of microfabrication- and soft lithography-based approaches; however, because these techniques rely on two-dimensional chemical patterning, they cannot be used to control cell-cell interactions at either the single cell- or tissue-level. By patterning non-adhesive and adhesive gels, we have engineered three-dimensional substrates that can control: 1) the exact location of cell-cell contacts in single cells with micrometer precision, and 2) the final architecture of multi-cellular aggregates. We will demonstrate how these substrates can be used to separate effects due to endothelial cell-cell contact from those due to diffusible, paracrine signaling. In addition, we will show how these techniques can be applied to create tissue constructs and stable, cell-based biosensor platforms.
Original language | English (US) |
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Pages (from-to) | 1700-1701 |
Number of pages | 2 |
Journal | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
Volume | 2 |
State | Published - 2002 |
Externally published | Yes |
Event | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002 |
All Science Journal Classification (ASJC) codes
- Signal Processing
- Biomedical Engineering
- Computer Vision and Pattern Recognition
- Health Informatics
Keywords
- Biosensors
- Cell and tissue engineering
- Microfabrication