TY - JOUR
T1 - Two-photon microscopy with simultaneous standard and extended depth of field using a tunable acoustic gradient-index lens
AU - Olivier, Nicolas
AU - Mermillod-Blondin, Alexandre
AU - Arnold, Craig B.
AU - Beaurepaire, Emmanuel
PY - 2009/6/1
Y1 - 2009/6/1
N2 - We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5×-extended depth-of-field imaging of developing embryos.
AB - We describe a simple setup that allows depth of field switching at kilohertz rates in a nonlinear microscope. Beam profile and/or divergence are modulated using a tunable, acoustically driven gradient-index fluid lens. We demonstrate two modulation strategies, one based on fast varifocus scanning during each pixel and the other based on pseudo-Bessel beam excitation. Average beam shape is switched every line during scanning, resulting in the interlaced acquisition of two different images. We apply this approach to the simultaneous standard and 4.5×-extended depth-of-field imaging of developing embryos.
UR - http://www.scopus.com/inward/record.url?scp=67649364233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649364233&partnerID=8YFLogxK
U2 - 10.1364/OL.34.001684
DO - 10.1364/OL.34.001684
M3 - Article
C2 - 19488148
AN - SCOPUS:67649364233
SN - 0146-9592
VL - 34
SP - 1684
EP - 1686
JO - Optics Letters
JF - Optics Letters
IS - 11
ER -