A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain’s microcircuitry is connected to animal behavior.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)