Two-photon imaging of neural activity in awake mobile mice

Daniel Dombeck; David Tank

doi:10.1101/pdb.top081810

Two-photon imaging of neural activity in awake mobile mice

Daniel Dombeck, David Tank

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain’s microcircuitry is connected to animal behavior.

Original language	English (US)
Pages (from-to)	726-736
Number of pages	11
Journal	Cold Spring Harbor Protocols
Volume	2014
Issue number	7
DOIs	https://doi.org/10.1101/pdb.top081810
State	Published - Jul 1 2014

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1101/pdb.top081810

Cite this

@article{cf6ee56b80f64e0a979e3b149003087a,

title = "Two-photon imaging of neural activity in awake mobile mice",

abstract = "A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain{\textquoteright}s microcircuitry is connected to animal behavior.",

author = "Daniel Dombeck and David Tank",

note = "Publisher Copyright: {\textcopyright} 2014 Cold Spring Harbor Laboratory Press.",

year = "2014",

month = jul,

day = "1",

doi = "10.1101/pdb.top081810",

language = "English (US)",

volume = "2014",

pages = "726--736",

journal = "Cold Spring Harbor Protocols",

issn = "1940-3402",

publisher = "Cold Spring Harbor Laboratory Press",

number = "7",

}

TY - JOUR

T1 - Two-photon imaging of neural activity in awake mobile mice

AU - Dombeck, Daniel

AU - Tank, David

PY - 2014/7/1

Y1 - 2014/7/1

N2 - A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain’s microcircuitry is connected to animal behavior.

AB - A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain’s microcircuitry is connected to animal behavior.

UR - http://www.scopus.com/inward/record.url?scp=84923798904&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923798904&partnerID=8YFLogxK

U2 - 10.1101/pdb.top081810

DO - 10.1101/pdb.top081810

M3 - Article

C2 - 24987148

AN - SCOPUS:84923798904

SN - 1940-3402

VL - 2014

SP - 726

EP - 736

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 7

ER -

Two-photon imaging of neural activity in awake mobile mice

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this