Two-photon imaging of neural activity in awake mobile mice

Daniel Dombeck, David Tank

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


A method for functional, cellular-resolution imaging of neural populations in awake and mobile mice is presented here. The method is based on the use of a spherical treadmill, head restraint, and motion correction software that facilitate neural imaging in the awake brain with a fixed upright two-photon microscope. These approaches have proven to be applicable to a wide range of brain regions and should help further our understanding of how neuronal population activity within the brain’s microcircuitry is connected to animal behavior.

Original languageEnglish (US)
Pages (from-to)726-736
Number of pages11
JournalCold Spring Harbor Protocols
Issue number7
StatePublished - Jul 1 2014

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology


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