TY - JOUR
T1 - Two hybrid plasmids with D. melanogaster DNA sequences complementary to mRNA coding for the major heat shock protein
AU - Schedl, Paul
AU - Artavanis-Tsakonas, Spyridon
AU - Steward, Ruth
AU - Gehring, Walter J.
AU - Mirault, Marc Edouard
AU - Goldschmidt-Clermont, Michel
AU - Moran, Larry
AU - Tissières, Alfred
N1 - Funding Information:
This work was supported by grants from the Swiss National Science Foundation (to W.J.G. and A.T.). P.S. is a postdoctoral fellow of the Helen Hay Whitney Foundation, and is supported by the Roche Research Foundation for Scientific Exchange and Biomedical Collaboration with Switzerland. S.A.-T. was supported by a long-term EM60 fellowship and the Swiss National Science Foundation (to W.J.G.). We thank M. Schedl. G. Martin, E. Wenger. M. Stempfel and J. Molliet for their assistance, and Mr. 0. Jenni for drawings and plates. We are also grateful to Dr. R. J. Jackson for his advice and gift of reticulocyte lysate. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.A. Section 1734 solely to indicate this fact.
PY - 1978/8
Y1 - 1978/8
N2 - The isolation and partial characterization of two cloned segments of Drosophila melanogaster DNA containing "heat shock" gene sequences is described. We have inserted sheared embryonic D. melanogaster DNA by the poly(dA-dT) connector method (Lobban and Kaiser, 1973) into the R1 restriction site of the ampicillin-resistant plasmid pSF2124 (So, Gill and Falkow, 1975). A collection of independent hybrid plasmids was screened by colony hybridization (Grunstein and Hogness, 1975) for sequences complementary to in vitro labeled polysomal poly(A)+ heat shock RNA. Two clones were identified which contain sequences complementary to a heat shock mRNA species that directs the in vitro synthesis of the 70,000 dalton heat-induced polypeptide. Both cloned segments hybridize in situ to the heat-induced puff sites located at 87A and 87C of the salivary gland polytene chromosomes.
AB - The isolation and partial characterization of two cloned segments of Drosophila melanogaster DNA containing "heat shock" gene sequences is described. We have inserted sheared embryonic D. melanogaster DNA by the poly(dA-dT) connector method (Lobban and Kaiser, 1973) into the R1 restriction site of the ampicillin-resistant plasmid pSF2124 (So, Gill and Falkow, 1975). A collection of independent hybrid plasmids was screened by colony hybridization (Grunstein and Hogness, 1975) for sequences complementary to in vitro labeled polysomal poly(A)+ heat shock RNA. Two clones were identified which contain sequences complementary to a heat shock mRNA species that directs the in vitro synthesis of the 70,000 dalton heat-induced polypeptide. Both cloned segments hybridize in situ to the heat-induced puff sites located at 87A and 87C of the salivary gland polytene chromosomes.
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U2 - 10.1016/0092-8674(78)90346-X
DO - 10.1016/0092-8674(78)90346-X
M3 - Article
C2 - 99246
AN - SCOPUS:0017897608
SN - 0092-8674
VL - 14
SP - 921
EP - 929
JO - Cell
JF - Cell
IS - 4
ER -