Two dimensional polyacrylamide gel electrophoresis of envelope proteins of Escherichia coli

W. C. Johnson, T. J. Silhavy, W. Boos

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A method of separating envelope proteins by two dimensional polyacrylamide gel electrophoresis is described. E. coli envelopes (inner and outer membranes) were prepared by French pressing and washed by repeated centrifugation. Membrane proteins were solubilized with guanidine thiocyanate and were dialyzed against urea prior to two dimensional electrophoretic analysis. The slab gel apparatus and conditions were similar to the technique developed by Metz and Bogorad for the separation of ribosomal proteins. This separation occurs in 8 M urea for the first dimension and in 0.2% sodium dodecyl sulfate for the second dimension. The technique separates about 70 different membrane proteins in a highly reproducible fashion according to both intrinsic charge and molecular weight. Some examples of alterations in the membrane protein pattern are demonstrated. These alterations are caused by a mutation affecting a sugar transport system and by growth in the presence of D fucose, inducer of the transport system. A further example of membrane protein changes introduced by growth at the nonpermissive temperature of a temperature sensitive cell division mutant is shown. Finally, it is demonstrated that the major outer membrane component of E. coli K 12 contains more than four proteins of similar molecular weight.

Original languageEnglish (US)
Pages (from-to)405-413
Number of pages9
JournalJournal of Applied Microbiology
Volume29
Issue number3
DOIs
StatePublished - Jan 1 1975
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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