TY - JOUR
T1 - Tumorigenicity of adenovirus-transformed cells
T2 - Region E1A of adenovirus 12 confers resistance to natural killer cells
AU - Sawada, Yukiharu
AU - Föhring, Bernd
AU - Shenk, Thomas E.
AU - Raška, Karel
N1 - Funding Information:
This work has been supported by Grants CA-21196 and CA-38965 from the National Cancer Institute. We thank Dr. Maurice Green and Dr. Arnold Levine for providing antibodies to Ad2 ElB proteins and Dr. Heinz Kunz for antibody to RT-1’ antigen. We gratefully acknowledge the excellent technical assistance of Ms. Janeen Dougherty and thank Ms. Bolusun Gutchinov for typing the manuscript.
PY - 1985/12
Y1 - 1985/12
N2 - Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12ElAB virus (containing E1A and E1B regions of Ad12) and with the sub370-12E1A virions (containing the E1A region of Ad12 and the E1B region of Ad5) showed a high degree of resistance to NK cells. The cell lines transformed with the sub370-12ElB virus (containing the E1A region of Ad5 and the E1B region of Ad12) were highly sensitive to NK cytotoxicity. While all cell lines transformed with virions containing the ElA genes of Ad5 expressed high levels of class I MHC antigen, only three of eight cell lines containing the E1A region of Ad12 showed detectable levels by flow cytometric analysis after staining with specific antibodies. All cell lines containing E1A genes of Ad5 were killed by in vitro generated allogeneic cytolytic T cells. Only three of eight cell lines containing the E1A region of Ad12 were killed by such CTLs; the level of cytotoxicity, however, did not reach that seen with the cells containing the E1A genes of Ad5. All cell lines containing the E1A and E1B genes of Ad12 were highly tumorigenic. Only two of four cell lines transformed with virus containing the E1A genes of Ad12 and ElB region of Ad5 were tumorigenic. The efficiency of tumor induction was low and the latent period was long confirming the importance of the ElB region. None of the cell lines transformed with virus containing the E1A region of Ad5 and the EIB genes of Ad12 were tumorigenic, reflecting their high degree of sensitivity to both natural and induced cellular immunity. Expression of the E1A region of Ad12 in transformed cells modulates not only the level of class I MHC antigens, but also confers resistance to NK cell cytotoxicity.
AB - Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12ElAB virus (containing E1A and E1B regions of Ad12) and with the sub370-12E1A virions (containing the E1A region of Ad12 and the E1B region of Ad5) showed a high degree of resistance to NK cells. The cell lines transformed with the sub370-12ElB virus (containing the E1A region of Ad5 and the E1B region of Ad12) were highly sensitive to NK cytotoxicity. While all cell lines transformed with virions containing the ElA genes of Ad5 expressed high levels of class I MHC antigen, only three of eight cell lines containing the E1A region of Ad12 showed detectable levels by flow cytometric analysis after staining with specific antibodies. All cell lines containing E1A genes of Ad5 were killed by in vitro generated allogeneic cytolytic T cells. Only three of eight cell lines containing the E1A region of Ad12 were killed by such CTLs; the level of cytotoxicity, however, did not reach that seen with the cells containing the E1A genes of Ad5. All cell lines containing the E1A and E1B genes of Ad12 were highly tumorigenic. Only two of four cell lines transformed with virus containing the E1A genes of Ad12 and ElB region of Ad5 were tumorigenic. The efficiency of tumor induction was low and the latent period was long confirming the importance of the ElB region. None of the cell lines transformed with virus containing the E1A region of Ad5 and the EIB genes of Ad12 were tumorigenic, reflecting their high degree of sensitivity to both natural and induced cellular immunity. Expression of the E1A region of Ad12 in transformed cells modulates not only the level of class I MHC antigens, but also confers resistance to NK cell cytotoxicity.
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U2 - 10.1016/0042-6822(85)90143-6
DO - 10.1016/0042-6822(85)90143-6
M3 - Article
C2 - 3878030
AN - SCOPUS:0022398995
SN - 0042-6822
VL - 147
SP - 413
EP - 421
JO - Virology
JF - Virology
IS - 2
ER -