Transient Electric Birefringence of Linear and Circular DNA: A Comparison of Kinetic Theory Predictions

John S. Bowers, Robert K. Prud'Homme

Research output: Contribution to journalArticlepeer-review

Abstract

The use of monodisperse DNA and restriction enzyme modification allows the preparation of model samples of polymer rings and linear chains with a precision that is not possible by conventional synthetic routes. These samples allow direct comparisons of the predictions of polymer kinetic theories. Transient electric birefringence allows rapid imposition or forces (∼100 ns) and monitoring of conformational changes (∼0.7 μs). We determined the relaxation spectra of once-cut linear φX-174 (5386 bp), twice-cut linear φX-174 (2693 bp), and relaxed ring (5386 bp) φX-174 with transient electric birefringence. This allows comparison of the relaxation dynamics of a linear chain and a polymer loop with exactly the same contour length. The relaxed loop and the twice-cut DNA had the longest relaxation times of 1039 and 1076 μs, respectively. The loop was measured both in the native supercoiled state and in the relaxed state. We found the birefringence decayed in agreement with bead-spring (Rouse/Zimm) models for polymer dynamics determined by both a model-independent average relaxation time and deconvolution of the decay into a sum of exponentials. Deconvolution was performed by CONTIN and by the Padé-Laplace method. For both the relaxed ring and the linear fragments, we found the longer time constants τ1 and τ2 were discrete and had a spacing that was in agreement with the Rouse model without hydrodynamic interaction (τ1/τ2 = 0.25), which would be expected for a chain with 20 statistical segments. The excitation of higher relaxation modes could be observed as the electric field pulse length increased.

Original languageEnglish (US)
Pages (from-to)8944-8952
Number of pages9
JournalJournal of Physical Chemistry B
Volume125
Issue number31
DOIs
StatePublished - Aug 12 2021

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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