TY - JOUR
T1 - Transformation by adenovirus early region 2A temperature-sensitive mutants and their revertants
AU - Logan, John
AU - Nicolas, Jean Claude
AU - Topp, William C.
AU - Girard, Marl
AU - Shenk, Thomas
AU - Levine, Arnold J.
N1 - Funding Information:
We acknowledge the competent technical assistance of Martha Marlow and Chris Paul. This work was supported by grants from the National Cancer Institute (CA28146 and CA24830) and the American Cancer Society (MV-47A).
PY - 1981/12
Y1 - 1981/12
N2 - H5ts107 is a temperature-sensitive mutant of adenovirus type 5 whose alteration maps in the structural gene for the viral DNA binding protein. Temperature-independent revertants of this mutant that form plaques at 39° in HeLa cells have been isolated. These revertants fall into two classes: (1) ts+ revertants for growth and plaque formation at 39° in both HeLa cells and 293 cells, a human cell line transformed by type 5 adenovirus; (2) ts+ for growth and plaque formation at 39° in HeLa cells, but temperature-sensitive (ts) for growth and plaque formation in 293 cells. The frequency of rat cell transformation by H5tsl07 was fivefold higher than that of wild-type adenovirus. A class 1 revertant, r(tsl07)127, transformed cells at the wild-type virus frequency while a class 2 revertant, r(tsl07)202, retained the ability to transform cells at the higher frequency. When the XhoI-C DNA fragment from these viruses, which contains the left end 15.5% of the viral genome, was employed to transform rat cells, then Ad5 wt, H5tsl07, r(tsl07)127, and r(ts107)202 DNAs all transformed with approximately equal frequencies.
AB - H5ts107 is a temperature-sensitive mutant of adenovirus type 5 whose alteration maps in the structural gene for the viral DNA binding protein. Temperature-independent revertants of this mutant that form plaques at 39° in HeLa cells have been isolated. These revertants fall into two classes: (1) ts+ revertants for growth and plaque formation at 39° in both HeLa cells and 293 cells, a human cell line transformed by type 5 adenovirus; (2) ts+ for growth and plaque formation at 39° in HeLa cells, but temperature-sensitive (ts) for growth and plaque formation in 293 cells. The frequency of rat cell transformation by H5tsl07 was fivefold higher than that of wild-type adenovirus. A class 1 revertant, r(tsl07)127, transformed cells at the wild-type virus frequency while a class 2 revertant, r(tsl07)202, retained the ability to transform cells at the higher frequency. When the XhoI-C DNA fragment from these viruses, which contains the left end 15.5% of the viral genome, was employed to transform rat cells, then Ad5 wt, H5tsl07, r(tsl07)127, and r(ts107)202 DNAs all transformed with approximately equal frequencies.
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U2 - 10.1016/0042-6822(81)90126-4
DO - 10.1016/0042-6822(81)90126-4
M3 - Article
C2 - 7314451
AN - SCOPUS:0019784248
SN - 0042-6822
VL - 115
SP - 419
EP - 422
JO - Virology
JF - Virology
IS - 2
ER -