TY - JOUR
T1 - Transformation by adenovirus early region 2A temperature-sensitive mutants and their revertants
AU - Logan, John
AU - Nicolas, Jean Claude
AU - Topp, William C.
AU - Girard, Marl
AU - Shenk, Thomas
AU - Levine, Arnold J.
PY - 1981/12
Y1 - 1981/12
N2 - H5ts107 is a temperature-sensitive mutant of adenovirus type 5 whose alteration maps in the structural gene for the viral DNA binding protein. Temperature-independent revertants of this mutant that form plaques at 39° in HeLa cells have been isolated. These revertants fall into two classes: (1) ts+ revertants for growth and plaque formation at 39° in both HeLa cells and 293 cells, a human cell line transformed by type 5 adenovirus; (2) ts+ for growth and plaque formation at 39° in HeLa cells, but temperature-sensitive (ts) for growth and plaque formation in 293 cells. The frequency of rat cell transformation by H5tsl07 was fivefold higher than that of wild-type adenovirus. A class 1 revertant, r(tsl07)127, transformed cells at the wild-type virus frequency while a class 2 revertant, r(tsl07)202, retained the ability to transform cells at the higher frequency. When the XhoI-C DNA fragment from these viruses, which contains the left end 15.5% of the viral genome, was employed to transform rat cells, then Ad5 wt, H5tsl07, r(tsl07)127, and r(ts107)202 DNAs all transformed with approximately equal frequencies.
AB - H5ts107 is a temperature-sensitive mutant of adenovirus type 5 whose alteration maps in the structural gene for the viral DNA binding protein. Temperature-independent revertants of this mutant that form plaques at 39° in HeLa cells have been isolated. These revertants fall into two classes: (1) ts+ revertants for growth and plaque formation at 39° in both HeLa cells and 293 cells, a human cell line transformed by type 5 adenovirus; (2) ts+ for growth and plaque formation at 39° in HeLa cells, but temperature-sensitive (ts) for growth and plaque formation in 293 cells. The frequency of rat cell transformation by H5tsl07 was fivefold higher than that of wild-type adenovirus. A class 1 revertant, r(tsl07)127, transformed cells at the wild-type virus frequency while a class 2 revertant, r(tsl07)202, retained the ability to transform cells at the higher frequency. When the XhoI-C DNA fragment from these viruses, which contains the left end 15.5% of the viral genome, was employed to transform rat cells, then Ad5 wt, H5tsl07, r(tsl07)127, and r(ts107)202 DNAs all transformed with approximately equal frequencies.
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U2 - 10.1016/0042-6822(81)90126-4
DO - 10.1016/0042-6822(81)90126-4
M3 - Article
C2 - 7314451
AN - SCOPUS:0019784248
VL - 115
SP - 419
EP - 422
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -