Transcriptionally regulated cell adhesion network dictates distal tip cell directionality

Ming Ching Wong, William P. Kennedy, Jean E. Schwarzbauer

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: The mechanisms that govern directional changes in cell migration are poorly understood. The migratory paths of two distal tip cells (DTC) determine the U-shape of the C. elegans hermaphroditic gonad. The morphogenesis of this organ provides a model system to identify genes necessary for the DTCs to execute two stereotyped turns. Results: Using candidate genes for RNAi knockdown in a DTC-specific strain, we identified two transcriptional regulators required for DTC turning: cbp-1, the CBP/p300 transcriptional coactivator homologue, and let-607, a CREBH transcription factor homologue. Further screening of potential target genes uncovered a network of integrin adhesion-related genes that have roles in turning and are dependent on cbp-1 and let-607 for expression. These genes include src-1/Src kinase, tln-1/talin, pat-2/α integrin and nmy-2, a nonmuscle myosin heavy chain. Conclusions: Transcriptional regulation by means of cbp-1 and let-607 is crucial for determining directional changes during DTC migration. These regulators coordinate a gene network that is necessary for integrin-mediated adhesion. Overall, these results suggest that directional changes in cell migration rely on the precise gene regulation of adhesion. Developmental Dynamics 243:999-1010, 2014.

Original languageEnglish (US)
Pages (from-to)999-1010
Number of pages12
JournalDevelopmental Dynamics
Volume243
Issue number8
DOIs
StatePublished - Aug 2014

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Keywords

  • C. elegans
  • Cell migration
  • Distal tip cell
  • Hermaphrodite gonadogenesis
  • Integrin

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