Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos

Amir Momen-Roknabadi, Stefano Di Talia, Eric Wieschaus

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25string in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same transcription factors that regulate the spatial pattern of cdc25string transcription encode its temporal activation. We identify buttonhead and empty spiracles as the major activators of cdc25string expression in mitotic domain 2. The effect of these activators is balanced through repression by hairy, sloppy paired 1, and huckebein. Within the mitotic domain, temporal precision of mitosis is robust and unaffected by changing dosage of rate-limiting transcriptional factors. However, precision can be disrupted by altering the levels of the two activators or two repressors. We propose that the additive and balanced action of activators and repressors is a general strategy for precise temporal regulation of cellular transitions during development.

Original languageEnglish (US)
Pages (from-to)2793-2801
Number of pages9
JournalCell Reports
Volume16
Issue number11
DOIs
StatePublished - Sep 13 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos'. Together they form a unique fingerprint.

Cite this