TY - JOUR
T1 - Transcriptional Timers Regulating Mitosis in Early Drosophila Embryos
AU - Momen-Roknabadi, Amir
AU - Di Talia, Stefano
AU - Wieschaus, Eric
N1 - Funding Information:
We thank the Drosophila Genomics Resource Center for plasmids and the Bloomington Drosophila Stock Center for fly stocks. We thank David Robinson for designing the statistical test. We thank Shelby Blythe and Reba Samanta for help with experiments. We thank Trudi Schüpbach, Paul Schedl, Ned Wingreen, William Bialek, Julie Merkle, Shawn Little, and E.W. and Schüpbach laboratory members for discussion. This work was supported by NIH grant R00-HD074670 (S.D.T.). E.W. is an investigator of the Howard Hughes Medical Institute.
Publisher Copyright:
© 2016 The Authors
PY - 2016/9/13
Y1 - 2016/9/13
N2 - The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25string in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same transcription factors that regulate the spatial pattern of cdc25string transcription encode its temporal activation. We identify buttonhead and empty spiracles as the major activators of cdc25string expression in mitotic domain 2. The effect of these activators is balanced through repression by hairy, sloppy paired 1, and huckebein. Within the mitotic domain, temporal precision of mitosis is robust and unaffected by changing dosage of rate-limiting transcriptional factors. However, precision can be disrupted by altering the levels of the two activators or two repressors. We propose that the additive and balanced action of activators and repressors is a general strategy for precise temporal regulation of cellular transitions during development.
AB - The development of an embryo requires precise spatiotemporal regulation of cellular processes. During Drosophila gastrulation, a precise temporal pattern of cell division is encoded through transcriptional regulation of cdc25string in 25 distinct mitotic domains. Using a genetic screen, we demonstrate that the same transcription factors that regulate the spatial pattern of cdc25string transcription encode its temporal activation. We identify buttonhead and empty spiracles as the major activators of cdc25string expression in mitotic domain 2. The effect of these activators is balanced through repression by hairy, sloppy paired 1, and huckebein. Within the mitotic domain, temporal precision of mitosis is robust and unaffected by changing dosage of rate-limiting transcriptional factors. However, precision can be disrupted by altering the levels of the two activators or two repressors. We propose that the additive and balanced action of activators and repressors is a general strategy for precise temporal regulation of cellular transitions during development.
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U2 - 10.1016/j.celrep.2016.08.034
DO - 10.1016/j.celrep.2016.08.034
M3 - Article
C2 - 27626650
AN - SCOPUS:84991619856
SN - 2211-1247
VL - 16
SP - 2793
EP - 2801
JO - Cell Reports
JF - Cell Reports
IS - 11
ER -