Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein

Yang Shi, Edward Seto, Long Sheng Chang, Thomas Shenk

Research output: Contribution to journalArticlepeer-review

921 Scopus citations

Abstract

A sequence within the transcription control region of the adeno-associated virus P5 promoter has been shown to mediate transcriptional activation by the adenovirus E1A protein. We report here that this same element mediates transcriptional repression in the absence of E1A. Two cellular proteins have been found to bind to overlapping regions within this sequence element. One of these proteins, YY1, is responsible for the repression. E1A relieves repression exerted by YY1 and further activates transcription through its binding site. A YY1-specific cDNA has been isolated. Its sequence reveals YY1 to be a zinc finger protein that belongs to the GLI-Krüppel gene family. The product of the cDNA binds to YY1 sites. When fused to the GAL4 DNA-binding domain, it is capable of repressing transcription directed by a promoter that contains GAL4-binding sites, and E1A proteins can relieve the repression and activate transcription through the fusion protein.

Original languageEnglish (US)
Pages (from-to)377-388
Number of pages12
JournalCell
Volume67
Issue number2
DOIs
StatePublished - Oct 18 1991

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology

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