@inbook{37406ceb84eb4ad1b4f617fe90dc964f,
title = "Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age",
abstract = "Multicellular organisms are composed of distinct cells and tissues that coordinate highly orchestrated responses to environmental challenges, including those that arise with age. Since C. elegans is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe a method for the FACS-based isolation and RNA sequencing of dissociated cells from adult C. elegans. This technique is amenable to profiling the cell- and tissue-specific gene expression changes in C. elegans mutants, including aging models, such as the daf-2/insulin-like signaling (IIS) pathway, and in wild-type animals with age.",
keywords = "Aging, Caenorhabditis elegans, Cell dissociation, FACS, RNA-seq, Transcriptional profiling",
author = "Rachel Kaletsky and Murphy, {Coleen T.}",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-0716-0592-9_16",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "177--186",
booktitle = "Methods in Molecular Biology",
}