Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age

Rachel Kaletsky, Coleen T. Murphy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multicellular organisms are composed of distinct cells and tissues that coordinate highly orchestrated responses to environmental challenges, including those that arise with age. Since C. elegans is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe a method for the FACS-based isolation and RNA sequencing of dissociated cells from adult C. elegans. This technique is amenable to profiling the cell- and tissue-specific gene expression changes in C. elegans mutants, including aging models, such as the daf-2/insulin-like signaling (IIS) pathway, and in wild-type animals with age.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages177-186
Number of pages10
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2144
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Aging
  • Caenorhabditis elegans
  • Cell dissociation
  • FACS
  • RNA-seq
  • Transcriptional profiling

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  • Cite this

    Kaletsky, R., & Murphy, C. T. (2020). Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age. In Methods in Molecular Biology (pp. 177-186). (Methods in Molecular Biology; Vol. 2144). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0592-9_16