Transcriptional occlusion caused by overlapping promoters

M. Ammar Zafar, Valerie J. Carabetta, Mark J. Mandel, Thomas J. Silhavy

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

RpoS (σ38) is required for cell survival under stress conditions, but it can inhibit growth if produced inappropriately and, consequently, its production and activity are elaborately regulated. Crl, a transcriptional activator that does not bind DNA, enhances RpoS activity by stimulating the interaction between RpoS and the core polymerase. The crl gene has two overlapping promoters, a housekeeping, RpoD- (σ70) dependent promoter, and an RpoN (σ54) promoter that is strongly up-regulated under nitrogen limitation. However, transcription from the RpoN promoter prevents transcription from the RpoD promoter, and the RpoN-dependent transcript lacks a ribosomebinding site. Thus, activation of the RpoN promoter produces a long noncoding RNA that silences crl gene expression simply by being made. This elegant and economical mechanism, which allows a near-instantaneous reduction in Crl synthesis without the need for transacting regulatory factors, restrains the activity of RpoS to allow faster growth under nitrogen-limiting conditions.

Original languageEnglish (US)
Pages (from-to)1557-1561
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number4
DOIs
StatePublished - Jan 28 2014

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • IncRNA
  • Transcriptional repression

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