Transcriptional Memory in the Drosophila Embryo

Teresa Ferraro, Emilia Esposito, Laure Mancini, Sam Ng, Tanguy Lucas, Mathieu Coppey, Nathalie Dostatni, Aleksandra M. Walczak, Michael Levine, Mounia Lagha

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Transmission of active transcriptional states from mother to daughter cells has the potential to foster precision in the gene expression programs underlying development. Such transcriptional memory has been specifically proposed to promote rapid reactivation of complex gene expression profiles after successive mitoses in Drosophila development [1]. By monitoring transcription in living Drosophila embryos, we provide the first evidence for transcriptional memory in animal development. We specifically monitored the activities of stochastically expressed transgenes in order to distinguish active and inactive mother cells and the behaviors of their daughter nuclei after mitosis. Quantitative analyses reveal that there is a 4-fold higher probability for rapid reactivation after mitosis when the mother experienced transcription. Moreover, memory nuclei activate transcription twice as fast as neighboring inactive mothers, thus leading to augmented levels of gene expression. We propose that transcriptional memory is a mechanism of precision, which helps coordinate gene activity during embryogenesis.

Original languageEnglish (US)
Pages (from-to)212-218
Number of pages7
JournalCurrent Biology
Volume26
Issue number2
DOIs
StatePublished - Jan 25 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Ferraro, T., Esposito, E., Mancini, L., Ng, S., Lucas, T., Coppey, M., Dostatni, N., Walczak, A. M., Levine, M., & Lagha, M. (2016). Transcriptional Memory in the Drosophila Embryo. Current Biology, 26(2), 212-218. https://doi.org/10.1016/j.cub.2015.11.058