Transcriptional interpretation of the EGF receptor signaling gradient

Alisa Fuchs, Lily S. Cheung, Enrica Charbonnier, Stanislav Y. Shvartsman, George Pyrowolakis

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Epidermal growth factor receptor (EGFR) controls a wide range of developmental events, from body axes specification in insects to cardiac development inhumans.During Drosophila oogenesis, a gradient of EGFR activation patterns the follicular epithelium. Multiple transcriptional targets of EGFR in this tissue have been identified, but their regulatory elements are essentially unknown. We report the regulatory elements of broad (br) and pipe (pip), two important targets of EGFR signaling in Drosophila oogenesis. br is expressed in a complex pattern that prefigures the formation of respiratory eggshell appendages. We found that this pattern is generated by dynamic activities of two regulatory elements, which display different responses to Pointed, Capicua, and Mirror, transcription factors involved in the EGFR-mediated gene expression. One of these elements is active in a pattern similar to pip, a gene repressed by EGFR and essential for establishing the dorsoventral polarity of the embryo. We demonstrate that this similarity of expression depends on a common sequence motif that binds Mirror in vitro and is essential for transcriptional repression in vivo.

Original languageEnglish (US)
Pages (from-to)1572-1577
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number5
DOIs
StatePublished - Jan 31 2012

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Follicle cells
  • Gene regulation
  • Patterning

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