Towards measurement of the time-resolved heat release of protein conformation dynamics

Jason Puchalla, Daniel Adamek, Robert Austin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We present a way to observe time-resolved heat release using a laminar flow diffusional mixer coupled with a highly sensitive infrared camera which measures the temperature change of the solvent. There are significant benefits to the use of laminar flow mixers for time-resolved calorimetry: (1) The thermal signal can be made position and time-stationary to allow for signal integration; (2) Extremely small volumes (nl/s) of sample are required for a measurement; (3) The same mixing environment can be observed spectroscopically to obtain state occupation information; (4) The mixer allows one to do out of equilibrium dynamic studies. The hope is that these measurements will allow us probe the non-equilibrium thermodynamics as a protein moves along a free energy trajectory from one state to another.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalChemical Physics
Volume307
Issue number2-3 SPEC.ISS.
DOIs
StatePublished - Dec 27 2004

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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