Tilted Brownian Ratchet for DNA Analysis

Lotien Richard Huang; Edward C. Cox; Robert H. Austin; James C. Sturm

doi:10.1021/ac0348524

Tilted Brownian Ratchet for DNA Analysis

Lotien Richard Huang, Edward C. Cox, Robert H. Austin, James C. Sturm

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90 Scopus citations

Abstract

In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of ∼100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in ∼70 min with a resolution of ∼3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.

Original language	English (US)
Pages (from-to)	6963-6967
Number of pages	5
Journal	Analytical Chemistry
Volume	75
Issue number	24
DOIs	https://doi.org/10.1021/ac0348524
State	Published - Dec 15 2003

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac0348524

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AB - In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of ∼100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in ∼70 min with a resolution of ∼3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.

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Tilted Brownian Ratchet for DNA Analysis

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