Tilted Brownian Ratchet for DNA Analysis

Lotien Richard Huang, Edward C. Cox, Robert H. Austin, James C. Sturm

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of ∼100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in ∼70 min with a resolution of ∼3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.

Original languageEnglish (US)
Pages (from-to)6963-6967
Number of pages5
JournalAnalytical Chemistry
Volume75
Issue number24
DOIs
StatePublished - Dec 15 2003

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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