TY - JOUR
T1 - Tilted Brownian Ratchet for DNA Analysis
AU - Huang, Lotien Richard
AU - Cox, Edward C.
AU - Austin, Robert H.
AU - Sturm, James C.
PY - 2003/12/15
Y1 - 2003/12/15
N2 - In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of ∼100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in ∼70 min with a resolution of ∼3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.
AB - In this paper, we report a factor of 3 improvement in the resolution and a factor of 10 improvement in the speed of fractionation of ∼100-kb DNA molecules in Brownian ratchet arrays. In our device, the electrophoretic flow is tilted at a small angle relative to the array axis. Tilting accelerates the fractionation speed because a higher fraction of the diffusing molecules is "ratcheted" at each step in the array. Molecules of lengths 48.5 and 164 kb can be separated in ∼70 min with a resolution of ∼3.8, using a 12-mm-long array. The Brownian ratchet arrays are not limited to DNA separation, but can, in principle, be used for any particle in this size range.
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U2 - 10.1021/ac0348524
DO - 10.1021/ac0348524
M3 - Article
C2 - 14670059
AN - SCOPUS:0348014619
SN - 0003-2700
VL - 75
SP - 6963
EP - 6967
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 24
ER -