Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

Katherine I. Swenson; Nica Borgese; Grazia Pietrini; Joan V. Ruderman

doi:10.1016/0012-1606(87)90421-0

Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

Katherine I. Swenson
, Nica Borgese
, Grazia Pietrini
, Joan V. Ruderman

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. ³H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.

Original language	English (US)
Pages (from-to)	10-16
Number of pages	7
Journal	Developmental biology
Volume	123
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(87)90421-0
State	Published - Sep 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology
Developmental Biology

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10.1016/0012-1606(87)90421-0

Cite this

@article{7f04809a1fd84110927c70abedf4d146,

title = "Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes",

abstract = "In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.",

author = "Swenson, \{Katherine I.\} and Nica Borgese and Grazia Pietrini and Ruderman, \{Joan V.\}",

note = "Funding Information: This work was supported by NSF Grant PCM 82-16917 to J.V.R., NIH postdoctoral fellowship F32 Al 06745-03-CLN to K.S., and an EMBO Fellowship to N.B. We are very grateful to Robert Angerer and Lynn Angerer for their very generous help the initial in situ hybridization experiments.",

year = "1987",

month = sep,

doi = "10.1016/0012-1606(87)90421-0",

language = "English (US)",

volume = "123",

pages = "10--16",

journal = "Developmental biology",

issn = "0012-1606",

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TY - JOUR

T1 - Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

AU - Swenson, Katherine I.

AU - Borgese, Nica

AU - Pietrini, Grazia

AU - Ruderman, Joan V.

N1 - Funding Information: This work was supported by NSF Grant PCM 82-16917 to J.V.R., NIH postdoctoral fellowship F32 Al 06745-03-CLN to K.S., and an EMBO Fellowship to N.B. We are very grateful to Robert Angerer and Lynn Angerer for their very generous help the initial in situ hybridization experiments.

PY - 1987/9

Y1 - 1987/9

N2 - In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.

AB - In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.

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U2 - 10.1016/0012-1606(87)90421-0

DO - 10.1016/0012-1606(87)90421-0

M3 - Article

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AN - SCOPUS:0023404120

SN - 0012-1606

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SP - 10

EP - 16

JO - Developmental biology

JF - Developmental biology

IS - 1

ER -

Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

Abstract

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