TY - JOUR
T1 - Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes
AU - Swenson, Katherine I.
AU - Borgese, Nica
AU - Pietrini, Grazia
AU - Ruderman, Joan V.
N1 - Funding Information:
This work was supported by NSF Grant PCM 82-16917 to J.V.R., NIH postdoctoral fellowship F32 Al 06745-03-CLN to K.S., and an EMBO Fellowship to N.B. We are very grateful to Robert Angerer and Lynn Angerer for their very generous help the initial in situ hybridization experiments.
PY - 1987/9
Y1 - 1987/9
N2 - In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.
AB - In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.
UR - http://www.scopus.com/inward/record.url?scp=0023404120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023404120&partnerID=8YFLogxK
U2 - 10.1016/0012-1606(87)90421-0
DO - 10.1016/0012-1606(87)90421-0
M3 - Article
C2 - 3040499
AN - SCOPUS:0023404120
SN - 0012-1606
VL - 123
SP - 10
EP - 16
JO - Developmental biology
JF - Developmental biology
IS - 1
ER -