Three translationally regulated mRNAs are stored in the cytoplasm of clam oocytes

Katherine I. Swenson, Nica Borgese, Grazia Pietrini, Joan V. Ruderman

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


In situ hybridization was used to examine the spatial distributions of three translationally controlled maternal RNAs in oocytes and two-cell embryos of the clam Spisula. 3H-labeled single-stranded RNA probes were generated from SP6 recombinant clones containing DNA inserts encoding portions of histone H3 (the DNA sequence which is presented here), cyclin A, and the small subunit of ribonucleotide reductase. Hybridization of these probes to oocytes, in which the mRNAs are translationally inactive, shows that these mRNAs are stored in the cytoplasm. There is no evidence for sequestration of any of the RNAs within the nucleus or any other discrete structure. Instead they appear to be evenly distributed throughout the cytoplasm.

Original languageEnglish (US)
Pages (from-to)10-16
Number of pages7
JournalDevelopmental biology
Issue number1
StatePublished - Sep 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology
  • Developmental Biology


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