Three-dimensional traction force microscopy of engineered epithelial tissues

Alexandra S. Piotrowski; Victor D. Varner; Nikolce Gjorevski; Celeste M. Nelson

doi:10.1007/978-1-4939-1164-6_13

Three-dimensional traction force microscopy of engineered epithelial tissues

Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, Celeste M. Nelson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

9 Scopus citations

Abstract

Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate the traction forces generated by engineered three-dimensional (3D) epithelial tissues embedded within a surrounding extracellular matrix (ECM). This technique provides insight into the physical mechanisms that underlie tissue morphogenesis in 3D.

Original language	English (US)
Title of host publication	Tissue Morphogenesis
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	191-206
Number of pages	16
ISBN (Electronic)	9781493911646
ISBN (Print)	9781493911639
DOIs	https://doi.org/10.1007/978-1-4939-1164-6_13
State	Published - Sep 22 2014

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
General Medicine

Keywords

Biomechanics
Engineered tissue
Micropatterning

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10.1007/978-1-4939-1164-6_13

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title = "Three-dimensional traction force microscopy of engineered epithelial tissues",

abstract = "Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate the traction forces generated by engineered three-dimensional (3D) epithelial tissues embedded within a surrounding extracellular matrix (ECM). This technique provides insight into the physical mechanisms that underlie tissue morphogenesis in 3D.",

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T1 - Three-dimensional traction force microscopy of engineered epithelial tissues

AU - Piotrowski, Alexandra S.

AU - Varner, Victor D.

AU - Gjorevski, Nikolce

AU - Nelson, Celeste M.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2014/9/22

Y1 - 2014/9/22

N2 - Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate the traction forces generated by engineered three-dimensional (3D) epithelial tissues embedded within a surrounding extracellular matrix (ECM). This technique provides insight into the physical mechanisms that underlie tissue morphogenesis in 3D.

AB - Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate the traction forces generated by engineered three-dimensional (3D) epithelial tissues embedded within a surrounding extracellular matrix (ECM). This technique provides insight into the physical mechanisms that underlie tissue morphogenesis in 3D.

KW - Biomechanics

KW - Engineered tissue

KW - Micropatterning

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M3 - Chapter

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SN - 9781493911639

SP - 191

EP - 206

BT - Tissue Morphogenesis

PB - Springer New York

ER -

Three-dimensional traction force microscopy of engineered epithelial tissues

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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