Three-dimensional particle tracking via tunable color-encoded multiplexing

Martí Duocastella, Christian Theriault, Craig B. Arnold

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


We present a novel 3D tracking approach capable of locating single particles with nanometric precision over wide axial ranges. Our method uses a fast acousto-optic liquid lens implemented in a bright field microscope to multiplex light based on color into different and selectable focal planes. By separating the red, green, and blue channels from an image captured with a color camera, information from up to three focal planes can be retrieved. Multiplane information from the particle diffraction rings enables precisely locating and tracking individual objects up to an axial range about 5 times larger than conventional single-plane approaches. We apply our method to the 3D visualization of the well-known coffee-stain phenomenon in evaporating water droplets.

Original languageEnglish (US)
Pages (from-to)863-866
Number of pages4
JournalOptics Letters
Issue number5
StatePublished - Mar 1 2016

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics


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