Three-dimensional deconvolution microfluidic microscopy using a tilted channel

Nicolas C. Pégard, Jason W. Fleischer

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We have developed a microfluidic device that enables the computation of three-dimensional (3-D) images of flowing samples. Using a microfluidic channel that is tilted along the optical axis, we record several progressively defocused images of the flowing sample as it passes across the focal plane. The resulting focal stack is then deconvolved to generate 3-D images. Experimental results on flowing yeast cells reveal both volume and surface profile information. The microfluidic channel eliminates the need for a precise translation stage to control defocusing and enables high sample throughput in an insulated, nontoxic, liquid environment. The experimental device can be implemented in all existing microscopes as a modified slide stage and is ideally suited for 3-D profiling in flow cytometers.

Original languageEnglish (US)
Article number040503
JournalJournal of Biomedical Optics
Volume18
Issue number4
DOIs
StatePublished - Jul 15 2013

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Keywords

  • cytometers
  • deconvolution
  • imaging
  • microfluidics
  • microscopy
  • optofluidics
  • profilometry
  • three-dimensional

Fingerprint Dive into the research topics of 'Three-dimensional deconvolution microfluidic microscopy using a tilted channel'. Together they form a unique fingerprint.

  • Cite this