Three different fibronectin mRNAs arise by alternative splicing within the coding region

Jean E. Schwarzbauer; John W. Tamkun; Ihor R. Lemischka; Richard O. Hynes

doi:10.1016/0092-8674(83)90175-7

Three different fibronectin mRNAs arise by alternative splicing within the coding region

Jean E. Schwarzbauer, John W. Tamkun, Ihor R. Lemischka, Richard O. Hynes

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Abstract

We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, λgt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.

Original language	English (US)
Pages (from-to)	421-431
Number of pages	11
Journal	Cell
Volume	35
Issue number	2 PART 1
DOIs	https://doi.org/10.1016/0092-8674(83)90175-7
State	Published - Dec 1983
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(83)90175-7

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@article{892acffbb3fa410aa9cc9dc49891f1e0,

title = "Three different fibronectin mRNAs arise by alternative splicing within the coding region",

abstract = "We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, λgt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.",

author = "Schwarzbauer, {Jean E.} and Tamkun, {John W.} and Lemischka, {Ihor R.} and Hynes, {Richard O.}",

note = "Funding Information: Thus research was supported by grants from the National Cancer Institute, USPHS. Jean E. Schwarzbauer was a recipient of a Damon Runyon-Walter Winchell postdoctoral fellowship. We are especially grateful to Richard Young for strarns and advice which allowed us to set up the hgtl 1 system. We would also like to thank John Gardner for his advice and suggestions on antibody blotting methods, Jeremy Paul for a helpful insight, Phil Sharp for useful discussrons, Jennifer Lee for her technical assistance, Richard Black for typing, and many other colleagues, too numerous to mention by name, who have given advice and support during{\textquoteright}the course of this project.",

year = "1983",

month = dec,

doi = "10.1016/0092-8674(83)90175-7",

language = "English (US)",

volume = "35",

pages = "421--431",

journal = "Cell",

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publisher = "Elsevier B.V.",

number = "2 PART 1",

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TY - JOUR

T1 - Three different fibronectin mRNAs arise by alternative splicing within the coding region

AU - Schwarzbauer, Jean E.

AU - Tamkun, John W.

AU - Lemischka, Ihor R.

AU - Hynes, Richard O.

N1 - Funding Information: Thus research was supported by grants from the National Cancer Institute, USPHS. Jean E. Schwarzbauer was a recipient of a Damon Runyon-Walter Winchell postdoctoral fellowship. We are especially grateful to Richard Young for strarns and advice which allowed us to set up the hgtl 1 system. We would also like to thank John Gardner for his advice and suggestions on antibody blotting methods, Jeremy Paul for a helpful insight, Phil Sharp for useful discussrons, Jennifer Lee for her technical assistance, Richard Black for typing, and many other colleagues, too numerous to mention by name, who have given advice and support during’the course of this project.

PY - 1983/12

Y1 - 1983/12

N2 - We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, λgt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.

AB - We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, λgt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.

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AN - SCOPUS:0021042241

SN - 0092-8674

VL - 35

SP - 421

EP - 431

JO - Cell

JF - Cell

IS - 2 PART 1

ER -

Three different fibronectin mRNAs arise by alternative splicing within the coding region

Abstract

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