Three different fibronectin mRNAs arise by alternative splicing within the coding region

Jean E. Schwarzbauer, John W. Tamkun, Ihor R. Lemischka, Richard O. Hynes

Research output: Contribution to journalArticle

473 Scopus citations

Abstract

We report the isolation of cDNA clones for fibronectin from a rat liver library prepared in the expression vector, λgt11. Restriction mapping and DNA sequencing of these clones establish the sequence of the C-terminal 35% of rat fibronectin, covering the cell-, heparin-, and fibrin-binding domains. The cell- and heparin-binding regions have homologous repeating sequences. Based on the sequence data and S1 nuclease mapping, we conclude that there are at least three different fibronectin mRNAs in rat liver which differ in coding potential. The three RNAs appear to arise by alternative splicing within the coding region and are probably all encoded by a single gene. The implications of these results for the structure and function of fibronectin and the differences between various types of fibronectin are discussed.

Original languageEnglish (US)
Pages (from-to)421-431
Number of pages11
JournalCell
Volume35
Issue number2 PART 1
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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