Thiol profiling in cancer cell lines by HPLC-mass spectrometry

Xiaoguang Liu; Pranavi Koppula; Kellen Olszewski; Boyi Gan

doi:10.1016/j.xpro.2021.100977

Thiol profiling in cancer cell lines by HPLC-mass spectrometry

Xiaoguang Liu, Pranavi Koppula, Kellen Olszewski, Boyi Gan

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).

Original language	English (US)
Article number	100977
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100977
State	Published - Dec 17 2021
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology
General Medicine

Keywords

Cancer
Mass Spectrometry
Metabolism

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10.1016/j.xpro.2021.100977

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title = "Thiol profiling in cancer cell lines by HPLC-mass spectrometry",

abstract = "We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).",

keywords = "Cancer, Mass Spectrometry, Metabolism",

author = "Xiaoguang Liu and Pranavi Koppula and Kellen Olszewski and Boyi Gan",

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AU - Liu, Xiaoguang

AU - Koppula, Pranavi

AU - Olszewski, Kellen

AU - Gan, Boyi

PY - 2021/12/17

Y1 - 2021/12/17

N2 - We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).

AB - We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).

KW - Cancer

KW - Mass Spectrometry

KW - Metabolism

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JO - STAR Protocols

JF - STAR Protocols

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M1 - 100977

ER -

Thiol profiling in cancer cell lines by HPLC-mass spectrometry

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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