Thiol profiling in cancer cell lines by HPLC-mass spectrometry

Xiaoguang Liu, Pranavi Koppula, Kellen Olszewski, Boyi Gan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).

Original languageEnglish (US)
Article number100977
JournalSTAR Protocols
Issue number4
StatePublished - Dec 17 2021
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine


  • Cancer
  • Mass Spectrometry
  • Metabolism


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