TY - JOUR
T1 - The transcription/migration interface in heart precursors of Ciona intestinalis
AU - Christiaen, Lionel
AU - Davidson, Brad
AU - Kawashima, Takeshi
AU - Powell, Weston
AU - Nolla, Hector
AU - Vranizan, Karen
AU - Levine, Michael
PY - 2008/6/6
Y1 - 2008/6/6
N2 - Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.
AB - Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.
UR - http://www.scopus.com/inward/record.url?scp=45549105130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45549105130&partnerID=8YFLogxK
U2 - 10.1126/science.1158170
DO - 10.1126/science.1158170
M3 - Article
C2 - 18535245
AN - SCOPUS:45549105130
SN - 0036-8075
VL - 320
SP - 1349
EP - 1352
JO - Science
JF - Science
IS - 5881
ER -