The temporal requirement for endothelin receptor-B signalling during neural crest development

Myung K. Shin, John M. Levorse, Robert S. Ingram, Shirley Marie Tilghman

Research output: Contribution to journalArticle

273 Scopus citations

Abstract

Endothelin receptor B (EDNRB) is a G-protein-coupled receptor with seven transmembrane domains which is required for the development of melanocytes and enteric neurons. Mice that are homozygous for a null mutation in the Ednrb gene are almost completely white and die as juveniles from megacolon. To determine when EDNRB signalling is required during embryogenesis, we have exploited the tetracycline-inducible system to generate strains of mice in which the endogenous Ednrb locus is under the control of the tetracycline- dependant transactivators tTa or rtTA. By using this system to express Ednrb at different stages of embryogenesis, we have determined that EDNRB is required during a restricted period of neural crest development between embryonic days 10 and 12.5. Moreover, our results imply that EDNRB is required for the migration of both melanoblasts and enteric neuroblasts.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalNature
Volume402
Issue number6761
DOIs
StatePublished - Dec 2 1999

All Science Journal Classification (ASJC) codes

  • General

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    Shin, M. K., Levorse, J. M., Ingram, R. S., & Tilghman, S. M. (1999). The temporal requirement for endothelin receptor-B signalling during neural crest development. Nature, 402(6761), 496-501. https://doi.org/10.1038/990040