The Spatiotemporal Limits of Developmental Erk Signaling

Heath E. Johnson, Yogesh Goyal, Nicole L. Pannucci, Trudi Schüpbach, Stanislav Y. Shvartsman, Jared E. Toettcher

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Animal development is characterized by signaling events that occur at precise locations and times within the embryo, but determining when and where such precision is needed for proper embryogenesis has been a long-standing challenge. Here we address this question for extracellular signal regulated kinase (Erk) signaling, a key developmental patterning cue. We describe an optogenetic system for activating Erk with high spatiotemporal precision in vivo. Implementing this system in Drosophila, we find that embryogenesis is remarkably robust to ectopic Erk signaling, except from 1 to 4 hr post-fertilization, when perturbing the spatial extent of Erk pathway activation leads to dramatic disruptions of patterning and morphogenesis. Later in development, the effects of ectopic signaling are buffered, at least in part, by combinatorial mechanisms. Our approach can be used to systematically probe the differential contributions of the Ras/Erk pathway and concurrent signals, leading to a more quantitative understanding of developmental signaling.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalDevelopmental cell
Volume40
Issue number2
DOIs
StatePublished - Jan 23 2017

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

Keywords

  • Drosophila
  • MAP kinase
  • embryogenesis
  • optogenetics
  • signal transduction

Fingerprint Dive into the research topics of 'The Spatiotemporal Limits of Developmental Erk Signaling'. Together they form a unique fingerprint.

Cite this