TY - JOUR
T1 - The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs
AU - Fitzgerald, Molly
AU - Shenk, Thomas
N1 - Funding Information:
We acknowledge the competent technical assistance of Martha Mar-low and the participation of Candace Swimmer in a part of this study. This work was supported by a USPHS research grant from the National Cancer Institute. T.S. is an established investigator of the American Heart Association. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1981/4
Y1 - 1981/4
N2 - We have observed three effects of deletion mutations on polyadenylation of late SV40 mRNAs. The first class of mutants lack segments (-3 to -14 bp) between the 5′-AAUAAA-3′ and normal poly(A) site. These mutants produce mRNAs polyadenylated at new sites, downstream from the wild-type site. The poly(A) site is moved farther downstream as the deletions become larger; as a result, polyadenylation always occurs within an 11-19 nucleotide range from the AAUAAA sequence. The second class of mutants lack segments (-12 to -30 bp) between the AAUAAA sequence and the coding region of the mRNA. The poly(A) site for only one of these mutants was studied (dl 1457, -12 bp). In this case, the spatial relationship between AAUAAA and poly(A) site is altered, dl1457 produces a class of mRNAs polyadenylated at the first CA following the AAUAAA sequence, as well as other mRNAs poly-adenylated farther downstream. Finally, a 16 bp deletion that includes the AAUAAA sequence prevents poly(A) addition.
AB - We have observed three effects of deletion mutations on polyadenylation of late SV40 mRNAs. The first class of mutants lack segments (-3 to -14 bp) between the 5′-AAUAAA-3′ and normal poly(A) site. These mutants produce mRNAs polyadenylated at new sites, downstream from the wild-type site. The poly(A) site is moved farther downstream as the deletions become larger; as a result, polyadenylation always occurs within an 11-19 nucleotide range from the AAUAAA sequence. The second class of mutants lack segments (-12 to -30 bp) between the AAUAAA sequence and the coding region of the mRNA. The poly(A) site for only one of these mutants was studied (dl 1457, -12 bp). In this case, the spatial relationship between AAUAAA and poly(A) site is altered, dl1457 produces a class of mRNAs polyadenylated at the first CA following the AAUAAA sequence, as well as other mRNAs poly-adenylated farther downstream. Finally, a 16 bp deletion that includes the AAUAAA sequence prevents poly(A) addition.
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U2 - 10.1016/0092-8674(81)90521-3
DO - 10.1016/0092-8674(81)90521-3
M3 - Article
C2 - 6113054
AN - SCOPUS:0019472442
SN - 0092-8674
VL - 24
SP - 251
EP - 260
JO - Cell
JF - Cell
IS - 1
ER -