The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs

Molly Fitzgerald, Thomas Shenk

Research output: Contribution to journalArticle

432 Scopus citations

Abstract

We have observed three effects of deletion mutations on polyadenylation of late SV40 mRNAs. The first class of mutants lack segments (-3 to -14 bp) between the 5′-AAUAAA-3′ and normal poly(A) site. These mutants produce mRNAs polyadenylated at new sites, downstream from the wild-type site. The poly(A) site is moved farther downstream as the deletions become larger; as a result, polyadenylation always occurs within an 11-19 nucleotide range from the AAUAAA sequence. The second class of mutants lack segments (-12 to -30 bp) between the AAUAAA sequence and the coding region of the mRNA. The poly(A) site for only one of these mutants was studied (dl 1457, -12 bp). In this case, the spatial relationship between AAUAAA and poly(A) site is altered, dl1457 produces a class of mRNAs polyadenylated at the first CA following the AAUAAA sequence, as well as other mRNAs poly-adenylated farther downstream. Finally, a 16 bp deletion that includes the AAUAAA sequence prevents poly(A) addition.

Original languageEnglish (US)
Pages (from-to)251-260
Number of pages10
JournalCell
Volume24
Issue number1
DOIs
StatePublished - Apr 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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