The secondary attachment site for bacteriophage λ in the pro A B gene of Escherichia coli

Jennifer L. Pinkham; Terry Platt; Lynn W. Enquist; Robert A. Weisberg

doi:10.1016/0022-2836(80)90339-3

The secondary attachment site for bacteriophage λ in the pro A B gene of Escherichia coli

Jennifer L. Pinkham, Terry Platt, Lynn W. Enquist, Robert A. Weisberg

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We have determined the nucleotide sequence of a secondary λ attachment site in pro A B, a site that accounts for 3% of lysogens isolated from Escherichia coli strains deleted for the primary site. Direct sequence analysis of the transducing bacteriophages carrying the left and right att junctions, as well as the recombinant pro⁺ phage reveals that the pro A B site shares an 11-nucleotide interrupted homology with the core sequence of the primary site. We have compared the pro A Batt site with other secondary attachment sites to gain insights into the structural features important for λ integration.

Original language	English (US)
Pages (from-to)	587-592
Number of pages	6
Journal	Journal of Molecular Biology
Volume	144
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(80)90339-3
State	Published - Dec 25 1980

All Science Journal Classification (ASJC) codes

Molecular Biology
Biophysics
Structural Biology

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10.1016/0022-2836(80)90339-3

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title = "The secondary attachment site for bacteriophage λ in the pro A B gene of Escherichia coli",

abstract = "We have determined the nucleotide sequence of a secondary λ attachment site in pro A B, a site that accounts for 3% of lysogens isolated from Escherichia coli strains deleted for the primary site. Direct sequence analysis of the transducing bacteriophages carrying the left and right att junctions, as well as the recombinant pro+ phage reveals that the pro A B site shares an 11-nucleotide interrupted homology with the core sequence of the primary site. We have compared the pro A Batt site with other secondary attachment sites to gain insights into the structural features important for λ integration.",

author = "Pinkham, {Jennifer L.} and Terry Platt and Enquist, {Lynn W.} and Weisberg, {Robert A.}",

note = "Funding Information: We thank K. Mizuuchi for readingt he manuscript,M . Rosa for her generousg ift of MnZI and I{\textquoteright}. Zennerf or her experta nd patient typing assistance. This work W~CsuSp portedi n part by a National Institutes of Health grant (to T. P.) (GM22830) and a National Institutes of Health predoctoral training grant (to J. L. P.).",

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doi = "10.1016/0022-2836(80)90339-3",

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T1 - The secondary attachment site for bacteriophage λ in the pro A B gene of Escherichia coli

AU - Pinkham, Jennifer L.

AU - Platt, Terry

AU - Enquist, Lynn W.

AU - Weisberg, Robert A.

N1 - Funding Information: We thank K. Mizuuchi for readingt he manuscript,M . Rosa for her generousg ift of MnZI and I’. Zennerf or her experta nd patient typing assistance. This work W~CsuSp portedi n part by a National Institutes of Health grant (to T. P.) (GM22830) and a National Institutes of Health predoctoral training grant (to J. L. P.).

PY - 1980/12/25

Y1 - 1980/12/25

N2 - We have determined the nucleotide sequence of a secondary λ attachment site in pro A B, a site that accounts for 3% of lysogens isolated from Escherichia coli strains deleted for the primary site. Direct sequence analysis of the transducing bacteriophages carrying the left and right att junctions, as well as the recombinant pro+ phage reveals that the pro A B site shares an 11-nucleotide interrupted homology with the core sequence of the primary site. We have compared the pro A Batt site with other secondary attachment sites to gain insights into the structural features important for λ integration.

AB - We have determined the nucleotide sequence of a secondary λ attachment site in pro A B, a site that accounts for 3% of lysogens isolated from Escherichia coli strains deleted for the primary site. Direct sequence analysis of the transducing bacteriophages carrying the left and right att junctions, as well as the recombinant pro+ phage reveals that the pro A B site shares an 11-nucleotide interrupted homology with the core sequence of the primary site. We have compared the pro A Batt site with other secondary attachment sites to gain insights into the structural features important for λ integration.

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The secondary attachment site for bacteriophage λ in the pro A B gene of Escherichia coli

Abstract

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