The Saccharomyces telomere-binding protein Cdc13p interacts with both the catalytic subunit of DNA polymerase α and the telomerase-associated Est1 protein

Haiyan Qi, Virginia A. Zakian

Research output: Contribution to journalArticle

271 Scopus citations

Abstract

Saccharomyces telomeres consist of -350 bp of C1-3A/TG1-3 DNA. Most of this -350 bp is replicated by standard, semiconservative DNA replication. After conventional replication, the C1-3A strand is degraded to generate a long single strand TG1-3 tail that can serve as a substrate for telomerase. Cdc13p is a single strand TG1-3 DNA-binding protein that localizes to telomeres in vivo. Genetic data suggest that the Cdc13p has multiple roles in telomere replication. We used two hybrid analysis to demonstrate that Cdc13p interacted with both the catalytic subunit of DNA polymerase α, Pol1p, and the telomerase RNA-associated protein, Est1p. The association of these proteins was confirmed by biochemical analysis using full-length or nearly full-length proteins. Point mutations in either CDC13 or POL1 that reduced the Cdc13p-Pol1p interaction resulted in telomerase mediated telomere lengthening. Over-expression of the carboxyl terminus of Est1p partially suppressed the temperature sensitive lethality of a cdc13-1 strain. We propose that Cdc13p's interaction with Est1p promotes TG1-3 strand lengthening by telomerase and its interaction with Pol1p promotes C1-3A strand resynthesis by DNA polymerase α.

Original languageEnglish (US)
Pages (from-to)1777-1788
Number of pages12
JournalGenes and Development
Volume14
Issue number14
StatePublished - Jul 15 2000

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • CDC13
  • DNA polymerase
  • EST1
  • POL1
  • Telomerase
  • Telomere replication

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